Note:Ce produit doit être emballé avec des sacs de glace et expédié via FedEx ou UPS.
Il arrivera dans 7-10 jours. Les frais d'expédition sont inclus dans le prix du produit.
Composants et contenus principaux:
| Composants | ED2304001-01（24T） | ED2304001-02（50T） |
| Fluorescent RAP solution de réaction | 500μL per tube × 1 tube | 1200μL per tube × 1 tube |
| Contrôle positif | 50μL per tube × 1 tube | 200μL per tube × 1 tube |
| Contrôle négatif | 50μL per tube × 1 tube | 200μL per tube × 1 tube |
| Manuel | 1 | 1 |
Fonction et but:
Utilisé pour détecter les acides nucléiques cibles dans divers échantillons, y compris le sang total, sérum, ganglions lymphatiques, rate, muscles, et d'autres.
Posologie et détermination:
1 Instructions d'utilisation
1.1 Traitement des échantillons
Process the samples according to relevant standards and store the processed samples for later use.
1.2 Procédure expérimentale
1.2.1 Reagent Preparation Area
Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;
Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.
1.2.2 Zone de préparation des échantillons
Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.
1.2.3 Amplification Area
Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.
Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.
| Système de réaction | 25μL Reaction System | ||
| Signal Channel | FAM Channel for Collecting Fluorescent Signals | ||
| PCR Reaction Conditions | Stage | Conditions | Cycles |
| Pre-Denaturation | 95℃：3min | 1 | |
| RAP | 95℃：15seconde | 40 | |
| 60℃：30seconde | |||
2 Interprétation des résultats
2.1 Establishment of Experimental Conditions:
2.1.1 Positive Control: Valeur CT ≤ 30, showing clear exponential growth, displaying a typical “S” curve.
2.1.2 Contrôle négatif: Valeur CT > 38 or no Ct value, showing no clear exponential growth phase or plateau.
2.2 Criteria for Determination:
2.2.1 Positive: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.
2.2.2 Soupçonné: Sample detection result Ct value between 35 et 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 et 38 with clear exponential growth, c'est considéré comme positif; sinon, it’s negative.
2.2.3 Négatif: Sample detection result Ct value > 38 or no Ct value, indicating the absence of the target DNA in the sample.
Précautions:
- Before the experiment, Lisez soigneusement ce manuel du kit de réactif et suivez strictement les procédures opérationnelles.
- Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
- Sample handling should occur in a biosafety cabinet. Après l'expérience, soumettre tous les échantillons et matériaux utilisés pour une stérilisation à haute température.
- To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (zone de préparation des réactifs, zone de préparation des échantillons, zone d'amplification, etc.).
- When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
- This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
- To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; pour le transport à longue distance, use dry ice.
- Avoid repeated freezing and thawing of all reagents.
Caractéristiques: 24 T/box, 50 T/box
Storage and Shelf Life: Stocker à -20 ° C, away from light. Shelf life is 12 mois.
