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이내에 도착할 예정입니다 7-10 날. 배송비는 상품가격에 포함되어 있습니다.
주요 구성 요소 및 내용:
| 구성요소 | ED2304001-01（24T） | ED2304001-02（50T） |
| Fluorescent PCR 반응 솔루션 | 500μL per tube × 1 튜브 | 1200μL per tube × 1 튜브 |
| 양성 대조군 | 50μL per tube × 1 튜브 | 200μL per tube × 1 튜브 |
| 음성 대조군 | 50μL per tube × 1 튜브 | 200μL per tube × 1 튜브 |
| 수동 | 1 | 1 |
기능과 목적:
전혈을 포함한 다양한 샘플에서 표적 핵산을 검출하는 데 사용, 혈청, 림프절, 비장, 근육, 다른 사람.
복용량과 결정:
1 사용 지침
1.1 샘플 처리
Process the samples according to relevant standards and store the processed samples for later use.
1.2 실험적 절차
1.2.1 시약 준비 영역
Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;
Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.
1.2.2 샘플 준비 영역
Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.
1.2.3 Amplification Area
Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.
Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.
| 반응 시스템 | 25μL Reaction System | ||
| Signal Channel | FAM Channel for Collecting Fluorescent Signals | ||
| PCR 반응 조건 | Stage | 정황 | 사이클 |
| Pre-Denaturation | 95℃：3분 | 1 | |
| PCR | 95℃：15비서 | 40 | |
| 60℃：30비서 | |||
2 결과 해석
2.1 Establishment of Experimental Conditions:
2.1.1 Positive Control: Ct 값 ≤ 30, showing clear exponential growth, displaying a typical “에스” curve.
2.1.2 부정적인 제어: Ct 값 > 38 또는 CT 값이 없습니다, showing no clear exponential growth phase or plateau.
2.2 Criteria for Determination:
2.2.1 긍정적인: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.
2.2.2 의심 가는: Sample detection result Ct value between 35 그리고 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 그리고 38 with clear exponential growth, 긍정적으로 여겨진다; 그렇지 않으면, it’s negative.
2.2.3 부정적인: Sample detection result Ct value > 38 또는 CT 값이 없습니다, indicating the absence of the target DNA in the sample.
지침:
- Before the experiment, 이 시약 키트 매뉴얼을 신중하게 읽고 운영 절차를 엄격하게 따르십시오..
- Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
- Sample handling should occur in a biosafety cabinet. 실험 후, 고온 멸균에 사용되는 모든 샘플과 재료를 대상으로합니다.
- To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (시약 준비 영역, 샘플 준비 영역, 증폭 영역, 등.).
- When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
- This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
- To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; 장거리 운송을 위해, use dry ice.
- Avoid repeated freezing and thawing of all reagents.
명세서: 24 티/상자, 50 티/상자
Storage and Shelf Life: -20°C에서 보관, away from light. 유효 기간입니다 12 개월.
