Nota:Questo prodotto deve essere imballato con sacche di ghiaccio e spedito tramite FedEx o UPS.
Arriverà all'interno 7-10 giorni. La commissione di spedizione è inclusa nel prezzo del prodotto.
Main Components and Contents:
| Componenti | ED2304001-01（24T） | ED2304001-02（50T） |
| Fluorescent PCR soluzione di reazione | 500μL per tube × 1 tubo | 1200μL per tube × 1 tubo |
| Controllo positivo | 50μL per tube × 1 tubo | 200μL per tube × 1 tubo |
| Controllo negativo | 50μL per tube × 1 tubo | 200μL per tube × 1 tubo |
| Manuale | 1 | 1 |
Function and Purpose:
Used for detecting target nucleic acids in various samples including whole blood, siero, lymph nodes, spleen, muscles, e altri.
Dosage and Determination:
1 Usage Instructions
1.1 Elaborazione del campione
Process the samples according to relevant standards and store the processed samples for later use.
1.2 Procedura sperimentale
1.2.1 Reagent Preparation Area
Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;
Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.
1.2.2 Area di preparazione del campione
Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.
1.2.3 Amplification Area
Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.
Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.
| Reaction System | 25μL Reaction System | ||
| Signal Channel | FAM Channel for Collecting Fluorescent Signals | ||
| Condizioni di reazione PCR | Stage | Condizioni | Cycles |
| Pre-Denaturation | 95℃：3min | 1 | |
| PCR | 95℃：15sec | 40 | |
| 60℃：30sec | |||
2 Interpretazione del risultato
2.1 Establishment of Experimental Conditions:
2.1.1 Positive Control: Valore CT ≤ 30, showing clear exponential growth, displaying a typical “S” curve.
2.1.2 Negative Control: Valore CT > 38 o nessun valore CT, showing no clear exponential growth phase or plateau.
2.2 Criteria for Determination:
2.2.1 Positivo: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.
2.2.2 Sospettato: Sample detection result Ct value between 35 E 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 E 38 with clear exponential growth, è considerato positivo; Altrimenti, it’s negative.
2.2.3 Negativo: Sample detection result Ct value > 38 o nessun valore CT, indicating the absence of the target DNA in the sample.
Precauzioni:
- Before the experiment, carefully read through this reagent kit manual and strictly follow the operational procedures.
- Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
- Sample handling should occur in a biosafety cabinet. After the experiment, subject all samples and materials used to high-temperature sterilization.
- To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (zona di preparazione dei reagenti, sample preparation area, amplification area, eccetera.).
- When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
- This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
- To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; for long-distance transport, use dry ice.
- Avoid repeated freezing and thawing of all reagents.
Specifiche: 24 T/scatola, 50 T/scatola
Storage and Shelf Life: Store at -20°C, away from light. Shelf life is 12 mesi.
