注記:この商品はアイスバッグで梱包し、FedEx または UPS で発送する必要があります。.
以内に到着します 7-10 日々. 送料は商品代金に含まれております.
主なコンポーネントと内容物:
| コンポーネント | ED2304001-01（24T） | ED2304001-02（50T） |
| Fluorescent PCR 反応溶液 | 500μL per tube × 1 チューブ | 1200μL per tube × 1 チューブ |
| ポジティブコントロール | 50μL per tube × 1 チューブ | 200μL per tube × 1 チューブ |
| ネガティブコントロール | 50μL per tube × 1 チューブ | 200μL per tube × 1 チューブ |
| マニュアル | 1 | 1 |
機能と目的:
全血を含むさまざまなサンプル中の標的核酸の検出に使用されます。, 血清, リンパ節, 脾臓, 筋肉, その他.
用量と決定:
1 使用手順
1.1 サンプル処理
Process the samples according to relevant standards and store the processed samples for later use.
1.2 Experimental Procedure
1.2.1 Reagent Preparation Area
Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;
Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.
1.2.2 サンプル準備エリア
Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.
1.2.3 Amplification Area
Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.
Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.
| 反応システム | 25μL Reaction System | ||
| Signal Channel | FAM Channel for Collecting Fluorescent Signals | ||
| PCR Reaction Conditions | Stage | 条件 | サイクル |
| Pre-Denaturation | 95℃：3分 | 1 | |
| PCR | 95℃：15秒 | 40 | |
| 60℃：30秒 | |||
2 結果の解釈
2.1 Establishment of Experimental Conditions:
2.1.1 Positive Control: Ct値≦ 30, showing clear exponential growth, displaying a typical “S” curve.
2.1.2 ネガティブコントロール: Ct値 > 38 or no Ct value, showing no clear exponential growth phase or plateau.
2.2 Criteria for Determination:
2.2.1 ポジティブ: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.
2.2.2 疑わしい: Sample detection result Ct value between 35 そして 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 そして 38 with clear exponential growth, それはポジティブだと考えられています; さもないと, it’s negative.
2.2.3 ネガティブ: Sample detection result Ct value > 38 or no Ct value, indicating the absence of the target DNA in the sample.
予防:
- Before the experiment, この試薬キットの説明書をよく読み、操作手順に厳密に従ってください。.
- Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
- Sample handling should occur in a biosafety cabinet. 実験後, 使用するすべてのサンプルと材料を高温滅菌する.
- To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (試薬準備エリア, サンプル準備エリア, 増幅領域, 等).
- When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
- This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
- To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; 長距離輸送用, use dry ice.
- Avoid repeated freezing and thawing of all reagents.
仕様: 24 T/箱, 50 T/箱
Storage and Shelf Life: -20℃で保存, away from light. 保存期間はです 12 月.
