Universeller fluoreszierender quantitativer DNA -Amplifikationskit 24T (PCR -Sondenmethode)

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Main Components and Contents:

Function and Purpose:

Used for detecting target nucleic acids in various samples including whole blood, Serum, lymph nodes, spleen, muscles, und andere.

Dosage and Determination:

1 Usage Instructions

1.1 Probenverarbeitung

Process the samples according to relevant standards and store the processed samples for later use.

1.2 Versuchsverfahren

1.2.1 Reagent Preparation Area

Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;

Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.

1.2.2 Probenvorbereitungsbereich

Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.

1.2.3 Amplification Area

Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.

Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.

2 Ergebnisinterpretation

2.1 Establishment of Experimental Conditions:

2.1.1 Positive Control: Ct-Wert ≤ 30, showing clear exponential growth, displaying a typical „S“ curve.

2.1.2 Negativkontrolle: Ct-Wert > 38 or no Ct value, showing no clear exponential growth phase or plateau.

2.2 Criteria for Determination:

2.2.1 Positiv: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.

2.2.2 Vermutlich: Sample detection result Ct value between 35 Und 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 Und 38 with clear exponential growth, Es wird als positiv angesehen; ansonsten, it’s negative.

2.2.3 Negativ: Sample detection result Ct value > 38 or no Ct value, indicating the absence of the target DNA in the sample.

Vorsichtsmaßnahmen:

• Before the experiment, carefully read through this reagent kit manual and strictly follow the operational procedures.
• Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
• Sample handling should occur in a biosafety cabinet. After the experiment, subject all samples and materials used to high-temperature sterilization.
• To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (Bereich zur Vorbereitung der Reagenzien, sample preparation area, amplification area, usw.).
• When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
• This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
• To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; for long-distance transport, use dry ice.
• Avoid repeated freezing and thawing of all reagents.

Spezifikationen: 24 T/Kasten, 50 T/Kasten

Storage and Shelf Life: Store at -20°C, away from light. Shelf life is 12 Monate.