Kit amplifikasi DNA kuantitatif universal 24t (Metode probe PCR)

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Komponen dan isi utama:

Fungsi dan tujuan:

Digunakan untuk mendeteksi asam nukleat target dalam berbagai sampel termasuk darah utuh, serum, kelenjar getah bening, limpa, otot, dan lain-lain.

Dosis dan tekad:

1 Petunjuk Penggunaan

1.1 Pemrosesan Sampel

Process the samples according to relevant standards and store the processed samples for later use.

1.2 Experimental Procedure

1.2.1 Reagent Preparation Area

Remove the reagent kit, take out the required reagents for the experiment, thaw and mix thoroughly, then briefly centrifuge for 5 seconds to remove any liquid adhering to the tube walls;

Add 20μL of fluorescent PCR reaction solution into each PCR tube and transfer it to the sample preparation area.

1.2.2 Area Persiapan Sampel

Add 5μL each of the negative control, the nucleic acid of the sample to be tested, and positive control. Briefly centrifuge for 5 seconds and transfer to the amplification area.

1.2.3 Amplification Area

Place each PCR tube in the corresponding positions of the instrument’s sample slots and record the placement sequence.

Set the instrument’s nucleic acid amplification parameters according to the table below and perform the PCR amplification.

2 Interpretasi Hasil

2.1 Establishment of Experimental Conditions:

2.1.1 Positive Control: Nilai Ct ≤ 30, showing clear exponential growth, displaying a typical “S” curve.

2.1.2 Kontrol Negatif: nilai Ct > 38 or no Ct value, showing no clear exponential growth phase or plateau.

2.2 Criteria for Determination:

2.2.1 Positive: Sample detection result Ct value ≤ 35, displaying clear exponential growth, indicating the presence of the target DNA in the sample.

2.2.2 Tersangka: Sample detection result Ct value between 35 Dan 38; the sample should be retested. If the repeated experiment results in Ct values still between 35 Dan 38 with clear exponential growth, itu dianggap positif; jika tidak, it’s negative.

2.2.3 Negatif: Sample detection result Ct value > 38 or no Ct value, indicating the absence of the target DNA in the sample.

Tindakan pencegahan:

• Before the experiment, Bacalah dengan cermat manual kit reagen ini dan dengan ketat ikuti prosedur operasional.
• Store all reagents at the specified temperatures; detailed information is available on the reagent labels.
• Sample handling should occur in a biosafety cabinet. Setelah percobaan, Subjek Semua sampel dan bahan yang digunakan untuk sterilisasi suhu tinggi.
• To prevent cross-contamination, work in an environment as free from nucleases as possible, and segment the experimental process (Area Persiapan Reagen, Area persiapan sampel, Area amplifikasi, dll.).
• When preparing the PCR reaction system, try to avoid bubble formation. Before amplification, check if reaction tubes are tightly sealed to prevent leakage and contamination of the instrument.
• This kit can be used with standard nucleic acid extraction purification methods for sample testing, maintaining constant judgment parameters such as Ct values.
• To ensure accurate experimental results, samples should be freshly collected and transported at 2-8°C; untuk transportasi jarak jauh, use dry ice.
• Avoid repeated freezing and thawing of all reagents.

Spesifikasi: 24 T/box, 50 T/box

Storage and Shelf Life: Simpan pada suhu -20°C, away from light. Umur simpan adalah 12 bulan.