Kit per il dosaggio delle proteine ​​BCA Solarbio 50T

La BCA (Acido bicinconinico) Protein Assay Kit is a widely utilized colorimetric method for the quantification of protein concentrations in a sample. This assay relies on the reduction of Cu^2+ ions to Cu^1+ by proteins under alkaline conditions, forming a purple-colored complex with bicinchoninic acid. The intensity of the color is directly proportional to the protein concentration, enabling accurate quantification.

Here is a step-by-step guide on how to use a Proteina BCA Kit di analisi:

Materiali richiesti:

1. Kit per il dosaggio delle proteine ​​BCA
2. Protein standards (bovine serum albumin is commonly used)
3. Sample containing unknown protein concentrations
4. Micropiastra o cuvette
5. Micropipette e puntali
6. Lettore di micropiastre o spettrofotometro
7. Acqua distillata

Procedura:

1. Preparazione degli standard proteici:
   • Preparare una serie di standard proteici con concentrazioni note. Questi standard verranno utilizzati per creare una curva standard per la quantificazione. Common concentrations are 0, 0.125, 0.25, 0.5, 0.75, E 1.0 mg/ml.
2. Preparation of Sample:
   • Dilute your protein sample to a suitable concentration within the linear range of the assay. If needed, you may need to perform a preliminary dilution to ensure that the sample falls within the standard curve.
3. Preparazione del reagente di lavoro BCA:
   • Mix BCA Reagent A and BCA Reagent B in a 50:1 rapporto (typically provided in the kit). Garantire una miscelazione accurata.
4. Addition of Standards and Sample:
   • Pipette aliquots of the protein standards and the sample into separate wells of a microplate. Each standard and sample should be run in duplicate or triplicate.
5. Addition of BCA Working Reagent:
   • Add an appropriate volume of the BCA Working Reagent to each well containing standards and samples. Mescolare accuratamente.
6. Incubazione:
   • Incubate the microplate at an appropriate temperature (typically 37°C) per 30 minutes to allow the color reaction to occur.
7. Misurazione:
   • Measure the absorbance of each well at 562 nm utilizzando un lettore di micropiastre o uno spettrofotometro.
8. Creazione della curva standard:
   • Tracciare i valori di assorbanza degli standard rispetto alle loro concentrazioni note per creare una curva standard.
9. Quantification of Sample:
   • Use the standard curve to determine the protein concentration of your sample based on its absorbance.
10. Analisi dei dati:
    • Calculate the concentration of protein in the sample based on the standard curve. Most microplate readers have software that can assist in this analysis.

Recensioni dei clienti sui prodotti Solarbio per riferimento