- Product Description:
- Designed for the purification of high-molecular-weight genomic or mitochondrial DNA.
- Suitable for various blood samples.
- Sample Types:
- Whole blood
- Buffy coat
- Bone marrow
- Body fluids
- Purification Time: As little as 25 minutes.
- Features:
- Convenient and scalable purification procedure.
- Removes contaminants and enzyme inhibitors like proteins and divalent cations.
- Purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Specifications
| Features | Specifications |
| Main Functions | Isolation of total DNA from whole blood using the economic salt out method |
| Applications | PCR, enzyme digestion, Southern hybridization, etc. |
| Purification technology | Salting out method |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Animal tissue |
| Sample amount | Unlimited |
| Elution volume | ≥100μl |
| Time per run | Variation with sample amount |
Principles
- Lysis Process:
- Cells are lysed with an anionic detergent in the presence of a DNA stabilizer.
- The DNA stabilizer limits the activity of intracellular DNases and DNases in the environment.
- RNA Removal:
- RNA is eliminated by treatment with an RNA-digesting enzyme.
- Contaminant Removal:
- Other contaminants, like proteins, are removed by salt precipitation.
- DNA Recovery:
- Genomic DNA is recovered by precipitation with alcohol.
- The recovered DNA is dissolved in Buffer TE.
- Quality Assurance:
- Purified DNA typically exhibits an A260/A280 ratio between 1.7 and 1.9.
- DNA size can reach up to 200 kb.
Advantages
- Safe – without phenol-chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic, and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- High purity – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Kit Contents
| Contents | D331101 | D331102 | D331103 |
| Purification Volumes | 50 ml | 300 ml | 1000 ml |
| 10 x Buffer RBC | 20 ml | 100 ml | 3 x 100 ml |
| Buffer WTL | 60 ml | 350 ml | 1000 ml |
| Buffer PPS | 20 ml | 120 ml | 350 ml |
| RNase Solution | 300 µl | 1.8 ml | 6 ml |
| Buffer TE | 10 ml | 30 ml | 120 ml |
Storage and Stability
- RNase Solution Storage:
- Upon arrival, store at 2-8°C.
- Short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect performance.
- Remaining Kit Components:
- Dry components can be stored at room temperature (15-25°C).
- Stable for at least 18 months under these conditions.
- Entire Kit Storage:
- The entire kit can be stored at 2-8°C.
- Buffers should be redissolved before use if stored at this temperature.
- Ensure all buffers are at room temperature when used.
