MagPure Circulating DNA Rich Maxi Kit

$1,094.00$3,978.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

This kit is designed to rich and extract 100bp-500bp circulating DNA from 5 ml cell-free body fluids (such asplasma, serum), and remove fragments above 500bp. Machine reaction only takes 90 minutes. Magnetic-particle technology provides high-quality DNA that is suitable for direct use in downstream applications such as PCR and next generation sequencing.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation circulating DNA from 5ml plasma, serum, body fluids
ApplicationsqPCR, NGS, etc.
Purification technologyMagnetic beads technology
Process methodManual (centrifugation or vacuum)
Sample typeSerum, plasma
Sample amount5ml
Elution volume≥40μl
Time per run≤50 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by elution buffer.

Advantages

  • Economy – less than 50% of the price of Qiagen and other imported products
  • Automatic – without labour

Kit Contents

Contents129275012927200
Purification Times50200
MagPure Particles G20 ml80 ml
MagBind Particles (selection particles)14 ml58 ml
Selection Solution100 ml400 ml
Proteinase K300 mg1.2 g
Protease Dissolve Buffer25 ml100 ml
Buffer SDS(20%)15 ml60 ml
Buffer MLK300 ml3 x 450 ml
Buffer BST1225 ml2x 450 ml
Buffer MKW1225 ml2x 450 ml
Buffer MW2*50 ml2x 100 ml
Buffer AE10 ml30 ml

Storage and Stability

MagPure Particles G, MagBind Particles and Proteinase K should bestored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at roomtemperature (15–25°C) and are stable for at least 18 months underthese conditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Additional information

Weight0.7 kg
size

MagPure Circulating DNA Rich Maxi Kit 50 preps, MagPure Circulating DNA Rich Maxi Kit 200 preps

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