Introduction
- Target: Short DNA fragments (<1000bp) in the blood (tumor markers, fetal DNA) or urine.
- Efficiency: Purifies circulating nucleic acids from human biofluids (plasma, serum, urine).
- Applications: Extracted DNA is suitable for approved clinical diagnostic tests.
Benefits:
- Optimized for short DNA analysis.
- Works with various human fluids.
- A larger format processes more samples (potentially higher DNA yield).
- Directly usable in clinical tests.
This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, and body fluids using vacuum protocol |
| Applications | qPCR, liquid or solid chip analysis, hybridization SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (vacuum) |
| Sample type | Serum, plasma, and other cell-free fluid samples |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
Principle
This product offers a streamlined method for isolating DNA using silica column technology:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, while proteins and other impurities pass through.
- Washing: Wash buffers are passed through the column, removing unbound proteins and cellular debris that didn’t adhere to the silica.
- Elution: A low-salt buffer is used to elute (release) the purified DNA from the silica membrane. This disrupts the interaction between DNA and the silica, allowing the collection of purified DNA in a separate tube.
Key Advantages:
- Efficient Purification: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
- High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (PCR, etc.).
- Simplified Workflow: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Advantages
- High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
- High concentration – low elution volume, ensuring high nucleic acid concentration
- High purity – low alcohol binding method, completely removing inhibitor and protein pollution
- High recovery – DNA can be recovered at the level of PG by silica gel column purification
Kit Contents
| Contents | IVD3182 |
| Purification Times | 50 |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinase K | 540 mg |
| Protease Dissolve Buffer | 30 ml |
| Carrier RNA | 110 μg |
| Nuclease Free Water | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 ml Collection Tubes | 100 |
| Extender Tube | 50 |
| Vac-Connector | 50 |
Storage and Stability
- Proteinase K & RNA Carrier: Fridge (2-8°C) for best results.
- Other Kit Components: Room temperature (15-25°C) is good.
- Storing Cold? Warm buffers up before use.
Experiment Data
Reviews
There are no reviews yet.