MagPure FFPE DNA Kit

$199.00$373.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

This product is suitable for rapid extraction of DNA from FFPE sample, tissue, cells, blood, swabs, blood spots, semen and other clinical samples. This product uses size selection magnetic beads, which can selectively remove small sizes of DNA (100-300bp) from FFPE samples by adjusting the amount of binding solution, so as to improve the effective data volume of downstream NGS.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from FFPE using high bind beads
ApplicationsRT-PCR, northern blot, poly A purification, nucleic acid protection and in vitro translation, etc.
Purification technologyMagnetic beads technology
Process methodManual or automatic
Sample typeLarge quantities of solids
Sample amountAppropriate
Elution volume≥50μl
Time per run30 – 120 minutes

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • High yield – most optimal process, recovery up to 90%
  • Economy – less than 50% of the price of Qiagen and other imported products
  • Various operations – manual single tube/high-throughput or workstation
  • High purity – OD 260/280 : 1.7-1.9, OD 260/230 :1.5-2.0

Kit Contents

ContentsD632301BD632302B
Purification Times48 Preps96 Preps
MagBind Particles1.1 ml2 x 1.1 ml
RNase A10 mg20 mg
Proteinase K24 mg48 mg
Protease Dissolve Buffer3 ml6 ml
Buffer DPS60 ml100 ml
Buffer ATL15 ml30 ml
Buffer AL15 ml30 ml
Buffer BD*6 ml15 ml
Buffer BXW1*13 ml44 ml
Elution Buffer15 ml30 ml

Storage and Stability

RNase A, Proteinase K and MagBind Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Additional information

Weight0.7 kg
size

MagPure FFPE DNA Kit 48 preps, MagPure FFPE DNA Kit 96 preps

brand name

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