Solarbio Tissue Total Phosphorus Content Assay Kit

Tissue Total Phosphorus Content Assay Kit

Bilješka: Uzmite dva ili tri različita uzorka za predviđanje prije testa.

Instrument za otkrivanje: Spectrophotometer /Microplate reader

Mačka ne: BC2855

Veličina: 100T/96S

Komponente:

Reagens i: 20 ml × 1. Skladištenje na 4 ℃ (strong corrosive, strong oxidizing). Reagens ⅱ: 7 ml × 1. Skladištenje na 4 ℃.

Reagens iii: Powder×2. Storage at 4℃ and protected from light. Radno rješenje: Fully dissolved with 5 mL of distilled water, then add 2.5 mL of Regent II, Dobro promiješajte.

Standard: 1 ml × 1, 1 mmol/L inorganic phosphorus standard. Skladištenje na 4 ℃.

Opis proizvoda:

The form of phosphorus includes inorganic phosphorus and organic phosphorus. Inorganic phosphorus mainly refers to phosphate radical, which is involved in many kinds of metabolism, including energy metabolism, nucleic acid metabolism, protein phosphorylation, dephosphorylation, and so on. By measuring the content of total phosphorus and inorganic phosphorus, the utilization rate of phosphorus in crops can be understood, and the basis for rational fertilization can be provided.

After digestion, total phosphorus was converted into inorganic phosphorus. The molybdenum blue method is a classical method for determining the content of inorganic phosphorus. Under certain conditions, molybdenum blue and phosphate from a substance with a characteristic absorption peak at 660 NM. By measuring the light absorption of 660 NM, the inorganic phosphorus content can be calculated, and then the total phosphorus content in the tissue can be calculated.

Potreban materijal

Centrifuga, spectrophotometer/microplate reader, vodena kupelji, micro glass cuvette/96 well flat-bottom plate, PRIJENOS, destilirana voda, concentrated sulfuric acid.

Postupak:

Ja. Sample Extraction:

0.1g of sample with 1mL of concentrated sulfuric acid (cover tightly to prevent moisture loss) put in boiling water bath for 10 minuta. When the solution is black or brown, take it out. Add 200μL of Reagent I after cooling, Dobro promiješajte. Continue boiling (cover tightly) until the solution is transparent, then cool to room temperature, add 3.8mL of distilled water and mix well. Centrifugated at, 10000rpm and room temperature for 10 minuta, supernatant is used for test.

Ii. Determination procedure:

1. Preheat the spectrophotometer/ microplate reader for 30 min, prilagoditi valnu duljinu na 660 NM, Postavite nulu s destiliranom vodom.
2. Set the temperature of water bath to 40℃.
3. Dodajte reagense sa sljedećim popisom:

Ime reagensa (μL)	Prazna cijev (Ab)	Standard (NA)	Epruveta (KAO)
Standard		10
Supernatant			10
Destilirana voda	100	90	90
Reagens iii	100	100	100

Mix well, 40℃ water bath for 10 minuta, detect the absorbance at 660 nm after cooling at room temperature for 10 minuta. Record as AB, AS and AT respectively.

Izračunavanje:

Total phosphate (mmol/g fresh weight)= =[C×(NA- Ab)÷(KAO – Ab)]×V÷W

=0.005×(NA – Ab)÷(KAO – Ab)÷W

C: standard concentration, 1mmol/L; V: supernatant volume, 5 mL=0.005 L; W: Težina uzorka, g.

Bilješka:

1．Reagent III should be prepared before use and only be used the same day.

2．Before the determination, 1–2 samples shall be used for pre-test. If the absorption value is greater than 1.5, distilled water shall be used for corresponding dilution.

Eksperimentalni primjer:

1. Take 0.1g kidney according to the extraction procedure, centrifugally take it up and clean it, and then follow the measurement procedure. Use 96 well plate to calculate: AT= 0.191, AB= 0.051, AS= 0.282. Calculate the total phosphorus content according to the sample mass

Total phosphorus content (mmol/g mass) = = 0.005 × (A-AB) ÷ (AS-AB) ÷ W= 0.005×(0.191-0.051) ÷ (0.282-0.051) ÷ 0.1= 0.030 mmol/g mass.

2. Take 0.1g spleen according to the extraction procedure, centrifugally take the cleaning, and then follow the measurement procedure. Use 96 well plate to calculate: AT= 0.197, AB= 0.051, AS= 0.282, calculate the total phosphorus content according to the sample mass

Total phosphorus content (mmol/g mass) =0.005×(A-AB) ÷(AS-AB)÷W= 0.005 × (0.197-0.051) ÷ (0.282- 0.051) ÷ 0.1= 0.032 mmol/g mass.

Povezani proizvodi：

BC2860/BC2865 Serum Total Iron Binding Capacity(TIBC) Komplet za analizu

BC2810/BC2815 Blood Zinc Content Assay Kit

BC2820/BC2825 Water Mercury Ion(Hg2+) Komplet za ispitivanje sadržaja

BC2840/BC2845 Phosphate Content Assay Kit

Technical Specifications:

The detection limit: 0.0338 mmol/L Linear range: 0.625-8 mmol/L