Introduction
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA colonne de rotation to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Co-isolation DNA and RNA from a single sample (cellules, mouchoir doux, échantillon de plante) |
| Applications | RT-RAP, synthèse d'ADNc, PCR andsecond-generation sequencing, etc.. |
| Méthode de purification | Mini colonne d'essorage |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| Montant de l'échantillon | Soft Tissue: < 30mg, Cellule: 1 X 107, Plant: 50 – 100mg |
| Rendement | ADN: 2 – 20 µg, ARN: 5 – 50 µg |
Principe
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Avantages
- Haute qualité – high purity total RNA / DNA can be directly used in a variety of downstream applications
- Rapide – column method can complete the extraction of several samples in 30 minutes
- Sûr – no phenol chloroform extraction required
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Contenu du kit
| Contenu | R511102 | R511103 |
| Temps de purification | 50 Préparations | 250 Préparations |
| Mini-colonnes HiPure DNA | 50 | 250 |
| Mini-colonnes HiPure ARN | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Tampon RW1 | 30 ml | 150 ml |
| Tampon RW2* | 20 ml | 2 X 50 ml |
| Eau sans RNase | 10 ml | 30 ml |
| Tampon AE | 10 ml | 50 ml |
Stockage et stabilité
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions.
Commentaires
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