Introducción
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA columna de giro to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Detalles
Especificaciones
| Características | Especificaciones |
| Funciones principales | Co-isolation DNA and RNA from a single sample (células, soft tissue, plant sample) |
| Aplicaciones | RT-PCR, síntesis de ADNc, PCR andsecond-generation sequencing, etc.. |
| Método de purificación | Mini columna de centrifugado |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| Cantidad de muestra | Soft Tissue: < 30mg, Celúla: 1 X 107, Planta: 50 – 100mg |
| Producir | ADN: 2 – 20 µg, ARN: 5 – 50 µg |
Principio
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Las muestras primero se lisan y homogeneizan.. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. El ARN de alta calidad se eluye en tan solo 30 µl de agua utilizando el kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Ventajas
- Alta calidad – ARN total de alta pureza / DNA can be directly used in a variety of downstream applications
- Rápido – column method can complete the extraction of several samples in 30 minutos
- Seguro – no se requiere extracción con fenol cloroformo
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Contenido del kit
| Contenido | R511102 | R511103 |
| Tiempos de purificación | 50 preparativos | 250 preparativos |
| HiPure DNA Mini Columns | 50 | 250 |
| Minicolumnas de ARN HiPure | 50 | 250 |
| 2Tubos de recolección de ml | 100 | 2 X 250 |
| RLC tampón | 50 ml | 200 ml |
| Memoria intermedia DW1 | 30 ml | 150 ml |
| Memoria intermedia RW1 | 30 ml | 150 ml |
| Búfer RW2* | 20 ml | 2 X 50 ml |
| Agua libre de ARNasa | 10 ml | 30 ml |
| AE de búfer | 10 ml | 50 ml |
Almacenamiento y estabilidad
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) y son estables durante al menos 18 meses en estas condiciones.
Reseñas
Aún no hay reseñas.