Perkenalan
This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA kolom putar to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.
Detail
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Co-isolation DNA and RNA from a single sample (sel, soft tissue, plant sample) |
| Aplikasi | RT-PCR, sintesis cDNA, PCR andsecond-generation sequencing, dll.. |
| Metode pemurnian | Kolom putaran mini |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Soft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues |
| Jumlah sampel | Soft Tissue: < 30mg, Sel: 1 X 107, Plant: 50 – 100mg |
| Menghasilkan | DNA: 2 – 20 μg, RNA: 5 – 50 μg |
Prinsip
The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.
Keuntungan
- Kualitas tinggi – high purity total RNA / DNA can be directly used in a variety of downstream applications
- Cepat – column method can complete the extraction of several samples in 30 menit
- Aman – Tidak diperlukan ekstraksi fenol kloroform
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Isi Paket
| Isi | R511102 | R511103 |
| Waktu Pemurnian | 50 Persiapan | 250 Persiapan |
| HiPure DNA Mini Columns | 50 | 250 |
| Kolom Mini RNA HiPure | 50 | 250 |
| 2ml Tabung Koleksi | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Buffer DW1 | 30 ml | 150 ml |
| Buffer RW1 | 30 ml | 150 ml |
| Penyangga RW2* | 20 ml | 2 X 50 ml |
| Air Bebas RNase | 10 ml | 30 ml |
| Penyangga AE | 10 ml | 50 ml |
Penyimpanan dan Stabilitas
HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) dan setidaknya stabil 18 bulan dalam kondisi ini.
Ulasan
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