介紹
This product offers a fast and reliable method to isolate RNA, 包括miRNA, from a wide range of samples:
- 組織
- 細胞
- 血
- Other clinical samples
The extracted RNA is suitable for various downstream applications, 包括:
- RT-聚合酶鍊式反應 (Reverse Transcription PCR)
- Quantitative RT-PCR (實時螢光定量PCR)
- Viral RNA detection
- Other RNA-based analyses
This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.
規格
| 特徵 | 規格 |
| 主要功能 | Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent |
| 應用領域 | 逆轉錄聚合酶連鎖反應, cDNA合成, second-generation sequencing |
| 產品 | 核糖核酸, miRNA |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 臨床組織, 細胞, 淋巴球 |
| 樣品量 | 組織: <20 毫克
細胞: <5 X 106 |
| 屈服 | 2-50米G |
| 洗脫體積 | ≥30μl |
| 每次運行時間 | ≤25分鐘 |
原則
- 純化方法: Silica column purification is employed in this product for effective nucleic acid extraction.
- Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
- 樣品裂解和消化: The sample lysis process, coupled with proteinase K digestion, takes only 15 分分鐘, ensuring rapid processing.
- Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
- Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
- Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
- 洗脫: 最後, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.
優點
- 高效 DNA 去除 – One-step RNA萃取 可有效去除基因組DNA
- 高品質 – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- 快速地 – 整個提取只需要 15-25 分分鐘
- 無毒 – no toxic phenol-chloroform extraction is required in the extraction
套件內容
| 內容 | 體外診斷4121 |
| 淨化時間 | 50 準備工作 |
| HiPure DNA 迷你柱 Ⅱ | 50 |
| HiPure RNA 迷你柱 | 50 |
| 2毫升收集管 | 150 |
| 蛋白酶K | 24 毫克 |
| 蛋白酶溶解緩衝液 | 1.8 毫升 |
| 脫氧核糖核酸酶I | 600 微升 |
| 脫氧核糖核酸酶緩衝液 | 6 毫升 |
| 緩衝RTL | 40 毫升 |
| RNA 消化緩衝液 | 15 毫升 |
| 緩衝區RWC* | 20 毫升 |
| 緩衝器 RW2* | 20 毫升 |
| 無核酸酶水 | 10 毫升 |
儲存和穩定性
- 蛋白酶 K 儲存: Store Proteinase K at 2–8°C upon arrival. 短期儲存 (取決於 8 週) 在室溫下 (15–25°C) is acceptable without affecting its performance.
- DNase I Storage: Store DNase I at -20°C. 短期儲存 (取決於 1 week) 在室溫下 (15–25°C) 不影響其性能.
- Remaining Kit Components Storage: 其餘試劑盒成分可在室溫下保存 (15–25°C) 至少保持穩定 18 在這些條件下幾個月.
評論
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