Einführung
This product offers a fast and reliable method to isolate RNA, including miRNA, from a wide range of samples:
- Gewebe
- Zellen
- Blut
- Andere klinische Proben
The extracted RNA is suitable for various downstream applications, einschließlich:
- RT-PCR (Reverse Transcription PCR)
- Quantitative RT-PCR (qRT-PCR)
- Viral RNA detection
- Other RNA-based analyses
This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation of total RNA（miRNA）from tissue, and cell using two columns and DNase plus reagent |
| Anwendungen | RT-PCR, cDNA-Synthese, second-generation sequencing |
| Produkte | RNA, miRNA |
| Reinigungsmethode | Mini Spin-Säule |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Clinical tissues, Zellen, Lymphozyten |
| Probenmenge | Gewebe: <20 mg
Zellen: <5 X 106 |
| Ertrag | 2-50MG |
| Elutionsvolumen | ≥30 μl |
| Zeit pro Lauf | ≤25 minutes |
Prinzip
- Reinigungsmethode: Silica column purification is employed in this product for effective nucleic acid extraction.
- Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
- Probe Lyse und Verdauung: The sample lysis process, coupled with proteinase K digestion, takes only 15 Protokoll, ensuring rapid processing.
- Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
- Nukleinsäureadsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
- Waschenschritt: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
- Elution: Endlich, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.
Vorteile
- Efficient DNA removal – One-step RNA-Extraktion can effectively remove genomic DNA
- Gute Qualität – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Schnell – the whole extraction only takes 15-25 Protokoll
- Nontoxic – no toxic phenol-chloroform extraction is required in the extraction
Inhalt des Kits
| Inhalt | IVD4121 |
| Reinigungszeiten | 50 Vorbereitungen |
| HiPure DNA Mini Column Ⅱ | 50 |
| HiPure RNA Mini-Säulen | 50 |
| 2ml-Sammelröhrchen | 150 |
| Proteinase K | 24 mg |
| Protease-Auflösungspuffer | 1.8 ml |
| DNase I | 600 μl |
| DNase Buffer | 6 ml |
| Buffer RTL | 40 ml |
| RNA Digestion Buffer | 15 ml |
| Buffer RWC* | 20 ml |
| Puffer RW2* | 20 ml |
| Nuklease freies Wasser | 10 ml |
Lagerung und Stabilität
- Proteinase K Speicherung: Store Proteinase K at 2–8°C upon arrival. Kurzfristige Lagerung (bis zu 8 Wochen) bei Raumtemperatur (15–25°C) is acceptable without affecting its performance.
- DNase I Storage: Store DNase I at -20°C. Kurzfristige Lagerung (bis zu 1 Woche) bei Raumtemperatur (15–25°C) hat keinen Einfluss auf seine Leistung.
- Verbleibende Kit -Komponenten Speicherung: Die restlichen Kit-Komponenten können bei Raumtemperatur gelagert werden (15–25°C) und bleiben zumindest stabil 18 Monate unter diesen Bedingungen.
Rezensionen
Es gibt noch keine Rezensionen.