MagPure FFPE RNA Kit

$994.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

This kit is suitable for high-throughput extraction of total RNA from paraffin embedded tissue samples, including miRNA. The kit is based on superparamagnetic magnetic particle purification technology. The extraction process does not require the use of toxic phenolic chloroform extraction or time-consuming alcohol precipitation.The whole extraction process only takes 120 minutes. The obtained RNA can be directly used in RT-PCR. The high-throughput extraction can be realized by 96 hole magnetic frame and 96 holeo scillator, various nucleic acid extractors (such as KingFisherFle, MagMix32, MagMix 96) or cooperating with the pipette workstation (Hamilton, Tican) for automatic extraction.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total RNA from FFPE tissue
ApplicationsRT-PCR, cDNA synthesis, second generation sequencing
ProductsRNA, miRNA
Purification methodPolydisperse magnetic beads
Purification technologyMagnetic beads technology
Process methodManual or automatic
Adaptive instrumentNucleic acid extractor, pipetting workstation
Sample typeFFPE slice, FFPE embedded tissue
Sample amount≤6 slices

Principle

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.

Advantages

  • Fast – several samples can be extracted in 120 minutes by column method
  • Safe – no phenol chloroform extraction required

Kit Contents

ContentsIVD3022
Purification Times200 Preps
MagBind Particles4.5 ml
Proteinase K100 mg
Protease Dissolve Buffer6 ml
DNase I4 x 600 µl
DNase Buffer30 ml
Buffer DPS200 ml
Buffer FRL40 ml
Buffer AL40 ml
Buffer MW1*110 ml
Buffer MW2*2 x 50 ml
Nuclease Free Water30 ml

Storage and Stability

Proteinase K and MagBind Particles should be stored at 2–8°C upon arrival. DNase I should bestored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K and MagBind Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.

Additional information

Weight0.7 kg

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