Introduction
The combination of improved CTAB extraction method and column purification technology is suitable for extracting high-purity RNA from various plant/fungal samples, especially plant tissues rich in polysaccharides and polyphenols (such as cotton leaves, mature rice leaves, Arabidopsis seeds, white pine needles, loquat leaves, roses, etc.). The obtained RNA can be directly used for experiments such as RT-PCR, Northern Blot, poly A+purification, nucleic acid protection, and in vitro translation. This product does not contain guanidine isothiocyanate throughout the entire process, and the purified RNA product does not contain guanidine isothiocyanate contamination, making A260/230 more stable.
Details
Kit Contents
| Contents | R4156 |
| Purification Times | 100 Preps |
| Column B10 | 2 x 100 |
| 2ml Collection Tubes | 2 x 100 |
| Buffer PAL | 100 ml |
| Buffer PRC1 | 60 ml |
| Buffer SW1 | 60 ml |
| Buffer SW2 | 110 ml |
| RNase Free Water | 20 ml |
Storage and Stability
The kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months.
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