HiPure Total RNA Plus Kit

$124.00$547.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

This kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total RNA (not include miRNA) from 20mg tissue, 150mg plant, 5 x 106 cell using two columns (gDNA removed column)
ApplicationsRT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification methodMini spin column
Purification technologySilica technology, DNA filtration technology
Process methodManual (centrifugation or vacuum)
Sample typeAnimal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amountCells:≤1 x 107

Animal tissue sample: 1-20 mg

Plant tissue: 50-150 mg

Yield2-100μg
Elution volume≥50μl
Time per run≤25 minutes(1-24 samples)
Liquid carrying volume per column800µl
Binding yield of column100µg

Principle

The Kit isolates total RNA from up to 107 cells or 20 mg tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 mins. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to a RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.

Advantages

  • Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Fast – several samples can be extracted in 25 minutes by column method
  • Safe – no phenol chloroform extraction required
  • Sensitive – RNA can be recovered at the level of PG

Kit Contents

ContentsR411102D411103
Purification Times50 Preps250 Preps
HiPure DNA Mini Columns50250
HiPure RNA Mini Columns50250
2ml Collection Tubes1002 x 250
Buffer RLC50 ml200 ml
Buffer RW150 ml200 ml
Buffer RW2*12 ml2 x 50 ml
RNase Free Water10 ml30 ml

Storage and Stability

HiPureKit can be stored dry at room temperature (15-25°C) and are stable for at least18 months under these conditions. During shipment, crystals or precipitationmay form in the Buffer RLC. Dissolve by warming buffer to 37°C.

Additional information

Weight0.7 kg
size

HiPure Total RNA Plus Kit 50 preps, HiPure Total RNA Plus Kit 250 preps

brand name

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