HiPure Total RNA Kit

$348.00

Shipping USD 45 - Free over USD 300

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

This product is suitable for rapid extraction of RNA (include miRNA) from tissue, cells, blood, s and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR, test of virus DNA and so on.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total RNA(miRNA)from tissue, cell using two columns and DNase plus reagent
ApplicationsRT-PCR, cDNA synthesis, second generation sequencing
ProductsRNA, miRNA
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeClinical tissues, cells, lymphocytes
Sample amountTissue: <20 mg

Cells: <5 x 106

Yield2-50μg
Elution volume≥30μl
Time per run≤25 minutes

Principle

This product is based on silica column purification. Remove paraffin by Buffer DPS. Sample lysis withproteinase K digestion requires only 15 minutes. After lysis, samples are incubated at 80ºC for 15 minutes. Transfer to an adsorption column and RNA is adsorbed on the membrane, while protein is not adsorbed andis removed with filtration. After washing proteins and other impurities, RNA was finally eluted with low-saltbuffer.

Advantages

  • Efficient DNA removal – one step RNA extraction can effectively remove genomic DNA
  • High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
  • Fast – the whole extraction only takes 15-25 minutes
  • Nontoxic – no toxic phenol chloroform extraction is required in the extraction

Kit Contents

ContentsIVD4121
Purification Times50 Preps
HiPure DNA Mini Column Ⅱ50
HiPure RNA Mini Columns50
2ml Collection Tubes150
Proteinase K24 mg
Protease Dissolve Buffer1.8 ml
DNase I600 μl
DNase Buffer6 ml
Buffer RTL40 ml
RNA Digestion Buffer15 ml
Buffer RWC*20 ml
Buffer RW2*20 ml
Nuclease Free Water10 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Additional information

Weight0.65 kg
brand name

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