Rapid cDNA First Strand Synthesis Kit 25T

Product Overview

• Genomic DNA Reverse Transcription Kit:
  • Available in M-MLV (RNase H-) Reverse Transcriptase.
  • New reverse transcriptase obtained through multiple point mutations, greatly improving thermal stability and cDNA synthesis efficiency.
  • Suitable for two-step RT-qPCR detection.
• RNA Template Preparation:
  • Use 4xgDNA Clear Short-term treatment with Mix to completely remove residual genomic DNA contamination from RNA templates.
  • Ensures subsequent quantitative results are more reliable and simplifies qPCR primer design.
• Reverse Transcription Process:
  • Join 5xqRT directly Reverse Mix for immediate reverse transcription.
  • 5xqRT Reverse Mix contains all components required for reverse transcription (Buffer, dNTP Mix, Hiscript Reverse Transcriptase, RNase inhibitor, Random primers/Oligo dT primer mix).
  • 4xgDNA Clear Mix terminates gDNA while preserving cDNA integrity.
  • RNA template volume can be added up to 60% of the total reaction volume, suitable for low-concentration RNA templates.
  • Both 4xgDNA Clear Mix and 5xqRT Reverse Mix are stable at -20°C for easy storage and use.
• Optimization for qPCR:
  • Specially optimized for qPCR with optimized random primers/Oligo dT primer mix ratio.
  • Allows cDNA synthesis initiation from various regions of RNA transcripts, maximizing qPCR result reproducibility.
  • Compatible with SYBR Green and probe method qPCR, allowing selection based on experimental needs.
  • Reagents can be used together with SYBR Green Master Mix for high-performance gene expression analysis.

Pre-experiment preparation and important precautions:

• RNase free of 1 . 5 ml microcentrifuge tube or 0.2 ml PCR tube
• Water bath or PCR machine
• Ice
• RNA: High-quality intact RNA is crucial to obtain high-quality cDNA, please use electrophoresis to verify the integrity of RNA.