Introduction
This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:
- Sample Types: Plasma, Serum (1-6ml)
- Target: cfDNA molecules
- Downstream Applications:
- PCR (polymerase chain reaction)
- Real-time PCR
- Biochip analysis
- Next-generation sequencing (NGS)
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-6ml plasma, serum, body fluids |
| Applications | qPCR, NGS, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Serum, plasma |
| Sample amount | 1-6ml |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
Principle
This product utilizes innovative magnetic bead technology for efficient DNA extraction:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, releasing DNA into the lysate.
- Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
- Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
- Elution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.
Advantages
- Economy – less than 50% of the price of Qiagen and other imported products
- Automatic – without labor
- High purity – OD 260 / 280: 1.7-1.9, OD 260 / 230:1.5-2.0
Kit Contents
| Contents | IVD5435 |
| Purification Times | 50 |
| MagPure Particles F | 14 ml |
| Carrier RNA | 310 μg |
| Proteinase K | 240 mg |
| Protease Dissolve Buffer | 15 ml |
| Buffer SDS | 15 ml |
| Buffer MLK | 500 ml |
| Buffer MAW1 | 250 ml |
| Buffer MW2* | 50 ml |
| Elution Buffer | 60 ml |
Storage and Stability
Ideal Storage:
- Store Proteinase K, Carrier RNA, and MagPure Particles F in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
Acceptable Short-Term Storage:
- These components can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.
Storing Remaining Components:
- All other kit components can be stored dry at room temperature (15-25°C).
- They will remain stable for at least 18 months under these conditions.
Important Notes:
- If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
- Always ensure all buffers reach room temperature before using them for optimal results.
Experiment Data
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