HiPure Total RNA Kit 50T- IVD4121

Introduction

This product offers a fast and reliable method to isolate RNA, including miRNA, from a wide range of samples:

• Tissue
• Cells
• Blood
• Other clinical samples

The extracted RNA is suitable for various downstream applications, including:

• RT-PCR (Reverse Transcription PCR)
• Quantitative RT-PCR (qRT-PCR)
• Viral RNA detection
• Other RNA-based analyses

This highlights the product’s versatility and compatibility with various research needs in gene expression analysis and RNA detection.

Specifications

Principle

• Purification Method: Silica column purification is employed in this product for effective nucleic acid extraction.
• Paraffin Removal: Buffer DPS is utilized to remove paraffin from the samples prior to the purification process.
• Sample Lysis and Digestion: The sample lysis process, coupled with proteinase K digestion, takes only 15 minutes, ensuring rapid processing.
• Incubation Conditions: Following lysis, samples are subjected to a 15-minute incubation at 80°C to facilitate optimal nucleic acid release.
• Nucleic Acid Adsorption: Transfer of the lysed samples to an adsorption column allows RNA to adsorb onto the membrane selectively, while proteins remain unadsorbed and are removed through filtration.
• Washing Step: Proteins and other impurities are washed away from the membrane to enhance the purity of the extracted RNA.
• Elution: Finally, RNA is eluted from the membrane using a low-salt buffer, ensuring efficient recovery of high-quality RNA.

Advantages

• Efficient DNA removal – One-step RNA extraction can effectively remove genomic DNA
• High quality – one-step RNA extraction reagent combined with silica gel column can obtain the highest concentration
• Fast – the whole extraction only takes 15-25 minutes
• Nontoxic – no toxic phenol-chloroform extraction is required in the extraction

Kit Contents

Storage and Stability

• Proteinase K Storage: Store Proteinase K at 2–8°C upon arrival. Short-term storage (up to 8 weeks) at room temperature (15–25°C) is acceptable without affecting its performance.
• DNase I Storage: Store DNase I at -20°C. Short-term storage (up to 1 week) at room temperature (15–25°C) does not affect its performance.
• Remaining Kit Components Storage: The remaining kit components can be stored at room temperature (15–25°C) and remain stable for at least 18 months under these conditions.