HiPure Paxigene Blood RNA Kit

$99.00$373.00

Shipping USD 45 - Free over USD 300

Isolation total RNA from Paxgene RNA tubes

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

The Kit provides fast purification of high-quality RNA from paxigene Blood RNA Tube using silica-membrane spin columns. RNA purified using the HiPure System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation of total RNA from Paxgene RNA Tubes
ApplicationsRT-PCR, qPCR, Northern hybridization, NGS, nucleic acid protection, in vitro translation
Purification methodMini spin column
Purification technologySilica technology, DNA filtration technology
Process methodManual (centrifugation or vacuum)
Sample typeBlood in the preservation tube
Sample amount2.5ml
Elution volume30μl
Liquid carrying volume per column800µl
Binding yield of column100μg

Principle

The Kit is for the purification of total RNA from 2.5 ml human whole blood collected in a PAXgene Blood RNA Tube. Purification begins with a centrifugation step to pellet nucleic acids in the PAXgene Blood RNA Tube. The pellet is washed and resuspended, and incubated in optimized buffers together with proteinase K. DNA wash The lysate is passed through a DNA Mini column. Ethanol is added to adjust binding conditions, and the lysate is applied to a column.RNA is selectively bound to the silica membrane as contaminants pass through. The remaining contaminants are removed in several efficient wash steps. Between the first and second wash steps, the membrane is treated with DNase I to remove trace amounts of bound DNA. After the wash steps, RNA is eluted in elution buffer and heat-denatured.

Advantages

  • High quality – high purity total RNA can be directly used in various sensitive downstream applications
  • Fast – isolation of several samples can be completed in 40 minutes by using the column purification method
  • Safety – no phenol-chloroform extraction is required
  • Sensitive – direct lysis of blood, plasma, and other samples without separation of leukocytes

Kit Contents

ContentsR416802R416803
Purification Times10 Preps50 Preps
HiPure RNA Mini Columns I1050
gDNA Filter Mini Columns1050
2ml Collection Tubes30150
RNase Free Water60 ml250 ml
Buffer MBR110 ml30 ml
Buffer MBR25 ml15 ml
Buffer RW115 ml60 ml
Buffer RW2*6 ml20 ml
Proteinase K12 mg50 mg
Protease Dissolve Buffer1.8 ml5 ml
DNase I120 µl600 µl
DNase Buffer6 ml30 ml

Storage and Stability

Proteinase K should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Proteinase K up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Experiment Data

Additional information

Weight0.85 kg
size

HiPure Paxigene Blood RNA Kit 10 preps, HiPure Paxigene Blood RNA Kit 50 preps

brand name

Reviews (0)

Reviews

There are no reviews yet.

Be the first to review “HiPure Paxigene Blood RNA Kit”

Your email address will not be published. Required fields are marked *

Attachment

Shopping Cart
Scroll to Top