HiPure DNA/RNA/Protein Kit

$273.00$1,193.00

Shipping USD 45 - Free over USD 300

Co-isolation DNA/RNA/ protein from a single sample(cells, soft tissue, plant sample)

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

FeaturesSpecifications
Main FunctionsCo-isolation DNA and RNA /protein from a single sample (cells, soft tissue, plant sample)
ApplicationsSDS-PAGE electrophoresis and western blot, etc
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeCulture cells, animal tissues, plant fungi, yeast, bacteria and other samples
Sample amountCultured cells: < 10^7

Animal tissue: ≤ 20 mg

Plant samples: ≤ 150 mg

Yeast cells: 2 x 10^6 – 5 x 10^7

Principle

The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.

Advantages

  • High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
  • Fast – column method can complete the extraction of several samples in 30 minutes
  • Safe – no phenol chloroform extraction required
  • Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

Kit Contents

ContentsR521102R521103
Purification Times50 Preps250 Preps
HiPure DNA Mini Columns50250
HiPure RNA Mini Columns50250
2ml Collection Tubes1002 x 250
Buffer RLC50 ml200 ml
Buffer GW1*22 ml66 ml
Buffer RW150 ml200 ml
Buffer RW2*50 ml3 x 50  ml
RNase Free Water10 ml30 ml
Elution Buffer10 ml30 ml
Buffer ALO(5%SDS)10 ml30 ml

Storage and Stability

HiPure Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions. During shipment, crystals or precipitation may form in the Buffer RLC. Dissolve by warming buffer to 37°C.

Additional information

Weight0.75 kg
size

HiPure DNA/RNA/Protein Kit 50 preps, HiPure DNA/RNA/Protein Kit 250 preps

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