Introduction
HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (cfDNA) from human biofluids for clinical use:
- Targets:
- Tumor-derived cfDNA fragments and mRNAs in blood
- Fetal nucleic acids in maternal blood
- Sample Types:
- Human plasma
- Human serum
- Human urine (fresh or frozen once)
- Focus on Short DNA: Captures short nucleic acids (<1000bp) commonly found in biofluids.
- Efficient Purification: Effectively recovers cfDNA from various biofluids.
- Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (IVD) tests.
Benefits:
- Optimized for Short cfDNA: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
- Broad Sample Compatibility: Works with various human biofluids, offering flexibility in sample selection.
- Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
- Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.
This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, non-invasive prenatal testing (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, body fluids by spin |
| Applications | qPCR, liquid or solid chip analysis, hybridization, and SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation) |
| Sample type | Serum, plasma, and other cell-free fluid samples |
| Sample amount | 1-5ml |
| Elution volume | ≥50μl |
| Time per run | ≤70 minutes |
| Liquid carrying volume per column | 4ml |
| Binding yield of column | 1mg |
Principle
This product utilizes silica gel columns for efficient DNA extraction:
- Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
- Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
- Washing: Wash buffers to remove leftover proteins and cellular debris.
- Elution: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.
This method provides a valuable tool for researchers working in various fields that require purified DNA for analysis.
Advantages
- High yield – most optimal process, free DNA (>50bp) can be obtained to the maximum extent
- High concentration – low elution volume, ensuring high nucleic acid concentration
- High purity – low alcohol binding method, completely removing inhibitor and protein pollution
- High recovery – DNA can be recovered at the level of PG by silica gel column purification
Kit Contents
| Contents | D318203D |
| Purification Times | 50 Preps |
| Buffer ACL | 250 ml |
| Buffer ACB* | 300 ml |
| Buffer DCW1* | 22 ml |
| Buffer DCW2* | 10 ml |
| Proteinase K | 540 mg |
| Protease Dissolve Buffer | 30 ml |
| Carrier RNA | 110 μg |
| Nuclease Free Water | 20 ml |
| HiPure CFDNA Mini Columns | 50 |
| 2 ml Collection Tubes | 100 |
| Extender Tubes | 50 |
| Sealing O-Ringes | 50 |
| 50ml Collection Tube | 50 |
| Support Tube | 50 |
Storage and Stability
- Proteinase K & Carrier RNA: Fridge (2-8°C) for best results.
- Everything Else: Room temperature (15-25°C) is fine.
- Important: If storing cold, warm buffers before use.
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