HiPure DNA/RNA Kit

$224.00$995.00

Shipping USD 45 - Free over USD 300

Co-Isolation DNA and RNA from a single sample (cells, soft tissue, plant sample)

DTE is a China-based e-commerce platform specializing in online sales of molecular testing, ELISA, and related products.

  • Manufacturer: Leading Chinese brands
  • Shipping: Expedited FedEx shipping directly from the factories
  • Eligible for return or replacement within 30 days
  • Payment Methods: Secure PayPal or credit card.

Description

Introduction

This Kit is designed to purify genomic DNA and total RNA simultaneously from a single biological sample. Lysate is first passed through a DNA spin column to selectively isolate DNA and then through an RNA column to selectively isolate RNA. Pure DNA and RNA are purified from the entire sample, in contrast to other procedures where either the biological sample or the purified total nucleic acids is divided into two before being processed separately. The kit is compatible with small amounts of a wide range of animal cells and tissues.

Details

Specifications

FeaturesSpecifications
Main FunctionsCo-isolation DNA and RNA from a single sample (cells, soft tissue, plant sample)
ApplicationsRT-PCR, cDNA synthesis, PCR andsecond-generation sequencing, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeSoft tissue samples (viscera, excluding skin andmuscle), cultured cells and common plant tissues
Sample amountSoft Tissue: < 30mg, Cell: 1 x 107, Plant: 50 – 100mg
YieldDNA: 2 – 20 μg,  RNA: 5 – 50 μg

Principle

The Kits are designed to purify both genomic DNA and total RNA from the same cellor tissue sample. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column and bind DNA. Ethanol is added to the flow-through and the sample is applied to an RNA column. DNA/RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit. High-quality DNA is eluted in as little as 50µl water using the Kit.

Advantages

  • High quality – high purity total RNA / DNA can be directly used in a variety of downstream applications
  • Fast – column method can complete the extraction of several samples in 30 minutes
  • Safe – no phenol chloroform extraction required
  • Simultaneous extraction- simultaneously isolate DNA and RNA from one sample

Kit Contents

ContentsR511102R511103
Purification Times50 Preps250 Preps
HiPure DNA Mini Columns50250
HiPure RNA Mini Columns50250
2ml Collection Tubes1002 x 250
Buffer RLC50 ml200 ml
Buffer DW130 ml150 ml
Buffer RW130 ml150 ml
Buffer RW2*20 ml2 x 50  ml
RNase Free Water10 ml30 ml
Buffer AE10 ml50 ml

Storage and Stability

HiPure DNA/RNA Kit can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

Additional information

Weight0.65 kg
size

HiPure DNA/RNA Kit 50 preps, HiPure DNA/RNA Kit 250 preps

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