바이러스 핵산 정제 시약 키트

1. 시약 키트의 구성 요소

2. 사용 지침:

1. The Virus Genome Purification Reagent Kit is intended for molecular biology research and should not be used for the prevention and treatment of diseases.
2. Prior to chemical handling, it is recommended to wear protective clothing, disposable gloves, and safety goggles.
3. Before using Rinse Buffer 1, 추가하다 (96%~100%) 에탄올.
4. The color change to yellow in Reagent Buffer C does not affect normal use.
5. Maintain the centrifugation environment at room temperature (15℃ ~ 25 ℃).

3. Reagent Kit Introduction:

The Virus Genome Purification Reagent Kit provides a rapid and effective solution for virus genome purification, widely applied to various viral nucleic acid purifications, such as Hepatitis B virus, 그리고 더.

The purification process can be completed within 1 시간, and the purified nucleic acids can be directly used for PCR, 서던 블로팅, 등. The entire purification process does not require toxic reagents such as phenol and chloroform, making the nucleic acid purification kit suitable for various other samples.

The purified RNA, washed with a low-salt solution or water, is suitable for downstream experiments. The A260/A280 ratio of the purified RNA is around 1.9, indicating high purity and a well-defined peak at 260 nm.

The high-efficiency membrane effectively removes inhibitors or other interfering substances, making it widely applicable to downstream experiments such as PCR, RAPD, AFLP, RFLP, SNP, 그리고 더.

4. Self-provided Equipment and Consumables:

High-speed centrifuge, EP 튜브, vortex oscillator, 무수에탄올, hot water bath or metal bath

5. Extraction Steps:

Preparation before starting:

ㅏ. Heat Elution Buffer to 65℃ before use (추천);

비. Add ethanol to씻다완충기 1 사용하기 전에.

1. 샘플 준비: Transfer 200μl of serum or other body fluids to a centrifuge tube. If insufficient, top up with PBS.
2. 추가하다 400시약 완충액의 μl c, 와류가 철저히, 실온에 잠시 놓아두세요 10 분, 와동 4-5 times during this period.
3. 추가하다 450μl of pre-cooled absolute ethanol, vortex immediately.
4. Transfer the obtained liquid to the RNA 추출 purification column (세트) (매번 약 650~700μl), 실온에 잠시 놓아두세요 2 분, 이상의 원심분리기 8,000 rpm 1 분, 수거된 폐기물을 폐기하다, 다음 단계를 위해 수집 튜브를 정제 컬럼에 다시 삽입합니다.
5. Place the RNA extraction purification column (세트) 새 수집 튜브에, 추가하다 300μl의 씻다 완충기 1, 이상의 원심분리기 8,000 rpm 1 분, 폐기물을 버리다, and re-insert the RNA extraction purification column (세트) 다음 단계를 위해 튜브에.

(메모: 절대 에탄올이 첨가되었음을 확인하십시오 씻다 완충기 1.)

6. 추가하다 700헹굼 완충액 μl 1 RNA 추출 정제 컬럼에 (세트), 원심분리기 14,000 rpm (20,000×g) ~을 위한 2 분, extend the centrifugation time appropriately to ensure the membrane is more dry.

(메모: 에탄올의 존재는 후속 실험에 심각한 영향을 미칩니다., 막을 건조시키는 것이 중요합니다. 원심분리 후, 용출 전에 에탄올이 존재하지 않는지 확인하십시오., then discard the waste and collection tube.)

7. Place the RNA extraction purification column (세트) 새로운 원심분리 튜브에, 똑똑 떨어지는 물방울 소리 100용출 버퍼의 μl 멤브레인 위에, 실온에서 배양하다 5 분 (15℃ ~ 25 ℃), 이상의 원심분리기 8,000 rpm 1 분.

(메모: Eluting with 50μl of elution buffer can increase nucleic acid concentration but reduces total nucleic acid yield.)

8. 이전 단계를 반복하세요..

(메모: A new centrifuge tube can be used to collect the nucleic acid eluted in the second round, or continue using the original collection tube to collect nucleic acid.)