Virus Nucleic Acid Purification Reagent Kit

1. Componenti del kit di reagenti

2. Istruzioni per l'uso:

1. The Virus Genome Purification Reagent Kit is intended for molecular biology research and should not be used for the prevention and treatment of diseases.
2. Prior to chemical handling, it is recommended to wear protective clothing, disposable gloves, and safety goggles.
3. Before using Rinse Buffer 1, aggiungere (96%~100%) etanolo.
4. The color change to yellow in Reagent Buffer C does not affect normal use.
5. Maintain the centrifugation environment at room temperature (15℃~25℃).

3. Reagent Kit Introduction:

The Virus Genome Purification Reagent Kit provides a rapid and effective solution for virus genome purification, widely applied to various viral nucleic acid purifications, such as Hepatitis B virus, e altro ancora.

The purification process can be completed within 1 ora, and the purified nucleic acids can be directly used for PCR, Southernblotting, eccetera. The entire purification process does not require toxic reagents such as phenol and chloroform, making the nucleic acid purification kit suitable for various other samples.

The purified RNA, washed with a low-salt solution or water, is suitable for downstream experiments. The A260/A280 ratio of the purified RNA is around 1.9, indicating high purity and a well-defined peak at 260 nm.

The high-efficiency membrane effectively removes inhibitors or other interfering substances, making it widely applicable to downstream experiments such as PCR, RAPD, AFLP, RFLP, SNP, e altro ancora.

4. Self-provided Equipment and Consumables:

High-speed centrifuge, EP tubes, vortex oscillator, absolute ethanol, hot water bath or metal bath

5. Extraction Steps:

Preparation before starting:

UN. Heat Elution Buffer to 65℃ before use (recommended);

B. Add ethanol toLavareRespingente 1 prima dell'uso.

1. Preparazione del campione: Transfer 200μl of serum or other body fluids to a centrifuge tube. If insufficient, top up with PBS.
2. Aggiungere 400μl of Reagent Buffer C, vortexare accuratamente, let it stand at room temperature for 10 minuti, vortex 4-5 times during this period.
3. Aggiungere 450μl of pre-cooled absolute ethanol, vortex immediately.
4. Transfer the obtained liquid to the Estrazione dell'RNA purification column (impostato) (circa 650~700μl ogni volta), let it stand at room temperature for 2 minuti, centrifuge at more than 8,000 giri al minuto per 1 minuto, scartare i rifiuti raccolti, e reinserire il tubo di raccolta nella colonna di purificazione per il passaggio successivo
5. Place the RNA extraction purification column (impostato) in una nuova provetta di raccolta, aggiungere 300ml di Lavare Respingente 1, centrifuge at more than 8,000 giri al minuto per 1 minuto, scartare i rifiuti, and re-insert the RNA extraction purification column (impostato) nel tubo per il passaggio successivo.

(Nota: Confirm that absolute ethanol has been added to Lavare Respingente 1.)

6. Aggiungere 700ml di tampone di risciacquo 1 to the RNA extraction purification column (impostato), centrifugare a 14,000 giri/min (20,000× g) per 2 minuti, extend the centrifugation time appropriately to ensure the membrane is more dry.

(Nota: The presence of ethanol has a serious impact on subsequent experiments, so drying the membrane is crucial. Dopo la centrifugazione, ensure no ethanol is present before elution, then discard the waste and collection tube.)

7. Place the RNA extraction purification column (impostato) in una nuova provetta da centrifuga, drip 100ml di tampone di eluizione sulla membrana, incubate at room temperature for 5 minuti (15℃~25℃), centrifuge at more than 8,000 giri al minuto per 1 minuto.

(Nota: Eluting with 50μl of elution buffer can increase nucleic acid concentration but reduces total nucleic acid yield.)

8. Repeat the previous step.

(Nota: A new centrifuge tube can be used to collect the nucleic acid eluted in the second round, or continue using the original collection tube to collect nucleic acid.)