Virus Nucleic Acid Purification Reagent Kit

1. Bestandteile des Reagenzienkits

2. Gebrauchsanweisung:

1. The Virus Genome Purification Reagent Kit is intended for molecular biology research and should not be used for the prevention and treatment of diseases.
2. Prior to chemical handling, it is recommended to wear protective clothing, disposable gloves, and safety goggles.
3. Before using Rinse Buffer 1, hinzufügen (96%~100%) Ethanol.
4. The color change to yellow in Reagent Buffer C does not affect normal use.
5. Maintain the centrifugation environment at room temperature (15℃~25℃).

3. Reagent Kit Introduction:

The Virus Genome Purification Reagent Kit provides a rapid and effective solution for virus genome purification, widely applied to various viral nucleic acid purifications, such as Hepatitis B virus, und mehr.

The purification process can be completed within 1 Stunde, and the purified nucleic acids can be directly used for PCR, Southern Blot, usw. The entire purification process does not require toxic reagents such as phenol and chloroform, making the nucleic acid purification kit suitable for various other samples.

The purified RNA, washed with a low-salt solution or water, is suitable for downstream experiments. The A260/A280 ratio of the purified RNA is around 1.9, indicating high purity and a well-defined peak at 260 nm.

The high-efficiency membrane effectively removes inhibitors or other interfering substances, making it widely applicable to downstream experiments such as PCR, RAPD, AFLP, RFLP, SNP, und mehr.

4. Self-provided Equipment and Consumables:

High-speed centrifuge, EP tubes, vortex oscillator, absolute ethanol, hot water bath or metal bath

5. Extraction Steps:

Preparation before starting:

A. Heat Elution Buffer to 65℃ before use (recommended);

B. Add ethanol toWaschenPuffer 1 vor dem Gebrauch.

1. Probenvorbereitung: Transfer 200μl of serum or other body fluids to a centrifuge tube. If insufficient, top up with PBS.
2. Hinzufügen 400μl Reagenzpuffer C., Vortex gründlich, let it stand at room temperature for 10 Protokoll, Wirbel 4-5 Zeiten in diesem Zeitraum.
3. Hinzufügen 450μl of pre-cooled absolute ethanol, vortex immediately.
4. Transfer the obtained liquid to the RNA-Extraktion purification column (Satz) (jedes Mal ungefähr 650 ~ 700 μl), let it stand at room temperature for 2 Protokoll, Zentrifuge um mehr als 8,000 U/min für 1 Minute, Entsorgen Sie den gesammelten Abfall, und setzen Sie das Sammelröhrchen für den nächsten Schritt wieder in die Reinigungssäule ein
5. Place the RNA extraction purification column (Satz) in einem neuen Sammelrohr, hinzufügen 300μl von Waschen Puffer 1, Zentrifuge um mehr als 8,000 U/min für 1 Minute, Entsorgen Sie den Abfall, and re-insert the RNA extraction purification column (Satz) in die Röhre für den nächsten Schritt.

(Notiz: Confirm that absolute ethanol has been added to Waschen Puffer 1.)

6. Hinzufügen 700μl Spülpuffer 1 to the RNA extraction purification column (Satz), Zentrifuge bei 14,000 U/min (20,000×g) für 2 Protokoll, extend the centrifugation time appropriately to ensure the membrane is more dry.

(Notiz: The presence of ethanol has a serious impact on subsequent experiments, so drying the membrane is crucial. Nach Zentrifugation, ensure no ethanol is present before elution, then discard the waste and collection tube.)

7. Place the RNA extraction purification column (Satz) in ein neues Zentrifugenröhrchen füllen, tropfen 100μl Elutionspuffer auf die Membran, bei Raumtemperatur inkubieren für 5 Protokoll (15℃~25℃), Zentrifuge um mehr als 8,000 U/min für 1 Minute.

(Notiz: Eluting with 50μl of elution buffer can increase nucleic acid concentration but reduces total nucleic acid yield.)

8. Wiederholen Sie den vorherigen Schritt.

(Notiz: A new centrifuge tube can be used to collect the nucleic acid eluted in the second round, or continue using the original collection tube to collect nucleic acid.)