- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 menit.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of kolom putar teknologi.
- Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
Spesifikasi
| Fitur | Spesifikasi |
| Fungsi utama | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 plate |
| Aplikasi | PCR, noda selatan dan pencernaan enzim, dll.. |
| Metode pemurnian | 96 piring sumur |
| Teknologi pemurnian | Teknologi silika |
| Metode proses | petunjuk (sentrifugasi atau vakum) |
| Jenis sampel | Tanah, stool, other environmental samples |
| Jumlah sampel | Tanah: 200-500mg
Bangku: 50-100mg Other environmental samples: 100-500mg |
| Volume elusi | |
| Waktu per lari | |
| Volume pembawa cairan per kolom | |
| Hasil pengikatan kolom |
Prinsip
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, ragi, and fungal samples.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, protein, polisakarida, and other contaminants from the lysed sample.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elution of Purified DNA: Pure DNA is eluted from the column in a low ionic strength buffer, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
Keuntungan
- Cepat – beberapa sampel dapat diekstraksi 40 menit (setelah pencernaan)
- Kemurnian tinggi – the purified DNA can be directly used in various downstream applications
- Pengulangan yang baik – teknologi silika dapat memperoleh hasil yang ideal setiap saat
- Pemulihan tinggi – DNA dapat dipulihkan pada tingkat PG
Isi Paket
| Isi | D314401 | D314402 | D314403 |
| Waktu Pemurnian | 1 X 96 Persiapan | 4 X 96 Persiapan | 20 X 96 Persiapan |
| Pelat DNA HiPure | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2tabung manik ML | 100 | 400 | 2000 |
| Buffer SOL | 100 ml | 360 ml | 2 X 900 ml |
| Penyangga SDS | 10 ml | 36 ml | 180 ml |
| Reagen DX | 1 ml | 1.8 ml | 9 ml |
| Penyangga PS | 20 ml | 80 ml | 400 ml |
| Absorber Solution | 20 ml | 80 ml | 400 ml |
| Buffer GDP | 150 ml | 500 ml | 3 X 900 ml |
| Penyangga GW2* | 50 ml | 100 ml | 4 X 200 ml |
| Penyangga AE | 30 ml | 120 ml | 500 ml |
Penyimpanan dan Stabilitas
- Absorber Solution Storage: Sesampainya disana, the Absorber Solution should be stored at 2-8°C. Namun, it can also be stored at room temperature (15-25°C) for short-term periods (hingga 24 minggu) without affecting its performance.
- Komponen Kit yang Tersisa: The remaining components of the kit can be stored dry at room temperature (15-25°C). They remain stable for at least 18 bulan dalam kondisi ini, ensuring their efficacy and performance over an extended period.
Ulasan
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