- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg de muestras de suelo dentro 60 minutos.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of columna de giro tecnología.
- Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, digestión de restricción, and next-generation sequencing.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
Especificaciones
| Características | Especificaciones |
| Funciones principales | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 lámina |
| Aplicaciones | PCR, Southern blot y digestión enzimática, etc.. |
| Método de purificación | 96 buen plato |
| Tecnología de purificación | tecnología de sílice |
| método de proceso | Manual (centrifugación o vacío) |
| Tipo de ejemplo | Suelo, stool, other environmental samples |
| Cantidad de muestra | Suelo: 200-500mg
Heces: 50-100mg Other environmental samples: 100-500mg |
| Volumen de elución | |
| Tiempo por carrera | |
| Volumen de transporte de líquido por columna | |
| Rendimiento de unión de la columna |
Principio
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, levadura, y muestras de hongos.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, proteínas, polisacáridos, y otros contaminantes de la muestra lisada.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elución de ADN purificado: El ADN puro se eluye de la columna en un tampón de resistencia iónica baja, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
Ventajas
- Rápido – Se pueden extraer varias muestras en 40 minutos (después de la digestión)
- Alta pureza – the purified DNA can be directly used in various downstream applications
- Buena repetibilidad – La tecnología de sílice puede obtener resultados ideales en todo momento.
- Alta recuperación – El ADN se puede recuperar a nivel de PG
Contenido del kit
| Contenido | D314401 | D314402 | D314403 |
| Tiempos de purificación | 1 X 96 preparativos | 4 X 96 preparativos | 20 X 96 preparativos |
| Placa de ADN HiPure | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6ml placa de recogida | 1 | 4 | 20 |
| 0.8ml placa de recogida | 1 | 4 | 20 |
| 2ML tubos de cuentas | 100 | 400 | 2000 |
| Buffer sol | 100 ml | 360 ml | 2 X 900 ml |
| Buffer Ficha de datos de seguridad (MSDS) | 10 ml | 36 ml | 180 ml |
| Reactivo DX | 1 ml | 1.8 ml | 9 ml |
| PS buffer | 20 ml | 80 ml | 400 ml |
| Solución absorbente | 20 ml | 80 ml | 400 ml |
| PIB de búfer | 150 ml | 500 ml | 3 X 900 ml |
| Tampón GW2* | 50 ml | 100 ml | 4 X 200 ml |
| AE de búfer | 30 ml | 120 ml | 500 ml |
Almacenamiento y estabilidad
- Absorber Solution Storage: A llegado, the Absorber Solution should be stored at 2-8°C. Sin embargo, it can also be stored at room temperature (15-25°C) for short-term periods (hasta 24 semanas) without affecting its performance.
- Componentes del kit restantes: The remaining components of the kit can be stored dry at room temperature (15-25°C). They remain stable for at least 18 meses en estas condiciones, ensuring their efficacy and performance over an extended period.
Reseñas
Aún no hay reseñas.