- Isolation of High-Quality Genomic DNA: The product enables rapid and reliable isolation of high-quality genomic DNA from various soil and stool samples.
- Sample Processing Capacity: Capable of processing up to 100mg of stool samples and 500 mg of soil samples within 60 Protokoll.
- Innovative System Design: Combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of Spin-Säule Technologie.
- Elimination of PCR Inhibitors: Effectively eliminates PCR-inhibiting compounds such as humic acid from soil samples during the purification process.
- Suitability for Various Applications: The purified DNA is suitable for PCR, Restriktionsverdauung, and next-generation sequencing.
- Reduction in Organic Extractions: No organic extractions are required, thus reducing plastic waste and hands-on time.
- Increased Efficiency: Allows for the processing of multiple samples in parallel, enhancing overall workflow efficiency.
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation DNA from 200-500mg soil, 50-100mg stool, or 100-500mg other environmental samples using 96 Platte |
| Anwendungen | PCR, southern blot and enzyme digestion, usw. |
| Reinigungsmethode | 96 Brunnenplatte |
| Reinigungstechnologie | Silica-Technologie |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Boden, stool, other environmental samples |
| Probenmenge | Boden: 200-500mg
Hocker: 50-100mg Other environmental samples: 100-500mg |
| Elutionsvolumen | |
| Zeit pro Lauf | |
| Flüssigkeitstransportvolumen pro Säule | |
| Bindungsausbeute der Säule |
Prinzip
- Sample Homogenization and Lysis: Soil or stool samples are homogenized and treated in a specially formulated buffer containing detergent. This process effectively lyses bacteria, Hefe, and fungal samples.
- Removal of Contaminants: Our proprietary Absorber Solution is utilized to remove humic acid, Proteine, Polysaccharide, und andere Verunreinigungen aus der lysierten Probe.
- DNA Binding and Column Application: After adjusting binding conditions, the treated sample is applied to a DNA Mini Column, facilitating the binding of DNA to the column matrix.
- Rapid Wash Steps: Two rapid wash steps are employed to remove trace contaminants, ensuring the purity of the extracted DNA.
- Elution der gereinigten DNA: Reine DNA wird aus der Säule in einem niedrigen Ionenstärkepuffer eluiert, providing high-quality, purified DNA ready for downstream applications.
- Direct Use in Downstream Applications: The purified DNA can be directly used in downstream applications without the need for further purification steps, streamlining laboratory workflows and saving time.
Vorteile
- Schnell – Es können mehrere Proben entnommen werden 40 Protokoll (after digestion)
- Hohe Reinheit – the purified DNA can be directly used in various downstream applications
- Gute Wiederholbarkeit – Die Silica -Technologie kann jedes Mal ideale Ergebnisse erzielen
- Hohe Genesung – DNA kann auf der Ebene von PG wiederhergestellt werden
Inhalt des Kits
| Inhalt | D314401 | D314402 | D314403 |
| Reinigungszeiten | 1 X 96 Vorbereitungen | 4 X 96 Vorbereitungen | 20 X 96 Vorbereitungen |
| HiPure DNA-Platte | 1 | 4 | 20 |
| 96 Well Plate (2.2ml) | 1 | 4 | 20 |
| 1.6ml Collection Plate | 1 | 4 | 20 |
| 0.8ml Collection Plate | 1 | 4 | 20 |
| 2ML Perlenröhrchen | 100 | 400 | 2000 |
| Buffer SOL | 100 ml | 360 ml | 2 X 900 ml |
| Puffer Sicherheitsdatenblatt | 10 ml | 36 ml | 180 ml |
| Reagent DX | 1 ml | 1.8 ml | 9 ml |
| Puffer PS | 20 ml | 80 ml | 400 ml |
| Absorber Solution | 20 ml | 80 ml | 400 ml |
| Puffer-BIP | 150 ml | 500 ml | 3 X 900 ml |
| Puffer GW2* | 50 ml | 100 ml | 4 X 200 ml |
| Puffer AE | 30 ml | 120 ml | 500 ml |
Lagerung und Stabilität
- Absorber Solution Storage: Bei der Ankunft, the Absorber Solution should be stored at 2-8°C. Jedoch, it can also be stored at room temperature (15-25°C) for short-term periods (bis zu 24 Wochen) without affecting its performance.
- Verbleibende Kit -Komponenten: The remaining components of the kit can be stored dry at room temperature (15-25°C). They remain stable for at least 18 Monate unter diesen Bedingungen, ensuring their efficacy and performance over an extended period.
Rezensionen
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