Virus Nucleic Acid Purification Reagent Kit

1. Composants du kit de réactifs

2. Mode d'emploi:

1. The Virus Genome Purification Reagent Kit is intended for molecular biology research and should not be used for the prevention and treatment of diseases.
2. Prior to chemical handling, it is recommended to wear protective clothing, disposable gloves, and safety goggles.
3. Before using Rinse Buffer 1, ajouter (96%~100%) éthanol.
4. The color change to yellow in Reagent Buffer C does not affect normal use.
5. Maintain the centrifugation environment at room temperature (15℃~25℃).

3. Reagent Kit Introduction:

The Virus Genome Purification Reagent Kit provides a rapid and effective solution for virus genome purification, widely applied to various viral nucleic acid purifications, such as Hepatitis B virus, et plus.

The purification process can be completed within 1 heure, and the purified nucleic acids can be directly used for RAP, Southern Blot, etc.. The entire purification process does not require toxic reagents such as phenol and chloroform, making the nucleic acid purification kit suitable for various other samples.

The purified RNA, washed with a low-salt solution or water, is suitable for downstream experiments. The A260/A280 ratio of the purified RNA is around 1.9, indicating high purity and a well-defined peak at 260 nm.

The high-efficiency membrane effectively removes inhibitors or other interfering substances, making it widely applicable to downstream experiments such as PCR, RAPD, AFLP, RFLP, SNP, et plus.

4. Self-provided Equipment and Consumables:

High-speed centrifuge, Tubes EP, vortex oscillator, éthanol absolu, hot water bath or metal bath

5. Extraction Steps:

Preparation before starting:

UN. Heat Elution Buffer to 65℃ before use (recommended);

B. Add ethanol toLaverTampon 1 Avant utilisation.

1. La préparation des échantillons: Transfer 200μl of serum or other body fluids to a centrifuge tube. If insufficient, top up with PBS.
2. Ajouter 400μl de tampon réactif C, vortexer soigneusement, Laissez-le se tenir à température ambiante pour 10 minutes, vortex 4-5 fois pendant cette période.
3. Ajouter 450μl of pre-cooled absolute ethanol, vortex immediately.
4. Transfer the obtained liquid to the Extraction d'ARN purification column (ensemble) (environ 650 ~ 700 μl à chaque fois), Laissez-le se tenir à température ambiante pour 2 minutes, centrifuger à plus de 8,000 tr/min pour 1 minute, jeter les déchets collectés, et réinsérez le tube de collecte dans la colonne de purification pour l'étape suivante
5. Place the RNA extraction purification column (ensemble) in a new collection tube, ajouter 300µl de Laver Tampon 1, centrifuger à plus de 8,000 tr/min pour 1 minute, jeter les déchets, and re-insert the RNA extraction purification column (ensemble) dans le tube pour la prochaine étape.

(Note: Confirm that absolute ethanol has been added to Laver Tampon 1.)

6. Ajouter 700µl de tampon de rinçage 1 to the RNA extraction purification column (ensemble), centrifuger à 14,000 tr/min (20,000×g) pour 2 minutes, extend the centrifugation time appropriately to ensure the membrane is more dry.

(Note: The presence of ethanol has a serious impact on subsequent experiments, so drying the membrane is crucial. Après centrifugation, s'assurer qu'aucun éthanol n'est présent avant l'élution, then discard the waste and collection tube.)

7. Place the RNA extraction purification column (ensemble) dans un nouveau tube à centrifuger, goutte 100µl de tampon d'élution sur la membrane, incuber à température ambiante pendant 5 minutes (15℃~25℃), centrifuger à plus de 8,000 tr/min pour 1 minute.

(Note: Eluting with 50μl of elution buffer can increase nucleic acid concentration but reduces total nucleic acid yield.)

8. Répétez l'étape précédente.

(Note: A new centrifuge tube can be used to collect the nucleic acid eluted in the second round, or continue using the original collection tube to collect nucleic acid.)