Virus Nucleic Acid Purification Reagent Kit

1. 試薬キットのコンポーネント

2. 使用説明書:

1. The Virus Genome Purification Reagent Kit is intended for molecular biology research and should not be used for the prevention and treatment of diseases.
2. Prior to chemical handling, it is recommended to wear protective clothing, disposable gloves, and safety goggles.
3. Before using Rinse Buffer 1, 追加 (96%~100%) エタノール.
4. The color change to yellow in Reagent Buffer C does not affect normal use.
5. Maintain the centrifugation environment at room temperature (15℃~25℃).

3. Reagent Kit Introduction:

The Virus Genome Purification Reagent Kit provides a rapid and effective solution for virus genome purification, widely applied to various viral nucleic acid purifications, such as Hepatitis B virus, などなど.

The purification process can be completed within 1 時間, and the purified nucleic acids can be directly used for PCR, サザンブロット, 等. The entire purification process does not require toxic reagents such as phenol and chloroform, making the nucleic acid purification kit suitable for various other samples.

The purified RNA, washed with a low-salt solution or water, is suitable for downstream experiments. The A260/A280 ratio of the purified RNA is around 1.9, indicating high purity and a well-defined peak at 260 nm.

The high-efficiency membrane effectively removes inhibitors or other interfering substances, making it widely applicable to downstream experiments such as PCR, RAPD, AFLP, RFLP, SNP, などなど.

4. Self-provided Equipment and Consumables:

High-speed centrifuge, EPチューブ, vortex oscillator, 絶対エタノール, hot water bath or metal bath

5. Extraction Steps:

Preparation before starting:

あ. Heat Elution Buffer to 65℃ before use (recommended);

B. Add ethanol toウォッシュバッファ 1 使用前に.

1. サンプルの準備: Transfer 200μl of serum or other body fluids to a centrifuge tube. If insufficient, top up with PBS.
2. 追加 400μl of Reagent Buffer C, vortex thoroughly, let it stand at room temperature for 10 分, vortex 4-5 この期間中の時間.
3. 追加 450μl of pre-cooled absolute ethanol, vortex immediately.
4. Transfer the obtained liquid to the RNA抽出 purification column (セット) (approximately 650~700μl each time), let it stand at room temperature for 2 分, 以上で遠心分離します 8,000 の回転数 1 分, 収集された廃棄物を廃棄します, and re-insert the collection tube into the purification column for the next step
5. Place the RNA extraction purification column (セット) in a new collection tube, 追加 300μlの ウォッシュ バッファ 1, 以上で遠心分離します 8,000 の回転数 1 分, 廃棄物を捨てます, and re-insert the RNA extraction purification column (セット) 次のステップのためにチューブに.

(注記: 絶対エタノールが追加されていることを確認してください ウォッシュ バッファ 1.)

6. 追加 700rinseバッファーのμl 1 to the RNA extraction purification column (セット), で遠心分離する 14,000 RPM (20,000×g) のために 2 分, extend the centrifugation time appropriately to ensure the membrane is more dry.

(注記: エタノールの存在は、その後の実験に深刻な影響を及ぼします, したがって、膜を乾燥させることが重要です. 遠心分離後, 溶出前にエタノールが存在しないことを確認してください, then discard the waste and collection tube.)

7. Place the RNA extraction purification column (セット) 新しい遠心チューブに, 滴下 100μLの溶出緩衝液 膜に, 室温でインキュベートします 5 分 (15℃~25℃), 以上で遠心分離します 8,000 の回転数 1 分.

(注記: Eluting with 50μl of elution buffer can increase nucleic acid concentration but reduces total nucleic acid yield.)

8. 前のステップを繰り返します.

(注記: A new centrifuge tube can be used to collect the nucleic acid eluted in the second round, or continue using the original collection tube to collect nucleic acid.)