Kit Maxi ADN circulant MagPure

Introduction

This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:

• Sample Types: Plasma, Sérum (1-6ml)
• Target: cfDNA molecules
• Applications en aval:
  • RAP (réaction en chaîne par polymérase)
  • Pcr en temps réel
  • Biochip analysis
  • Next-generation sequencing (NGS)

Caractéristiques

Principe

This product utilizes innovative magnetic bead technology for efficient DNA extraction:

1. Lyse et digestion: The sample undergoes treatment with a special solution (lysat) et une enzyme (protéase) to break open cells and degrade proteins, releasing DNA into the lysate.
2. Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
3. Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
4. Élution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, Permettre la collecte de l'ADN purifié dans un tube séparé.

Avantages

• Économie – moins que 50% du prix de Qiagen et d'autres produits importés
• Automatique – without labor
• Haute pureté – OD 260 / 280: 1.7-1.9, OD 260 / 230:1.5-2.0

Contenu du kit

Stockage et stabilité

Ideal Storage:

• Store Proteinase K, ARN porteur, and MagPure Particles F in the refrigerator (2-8°C) dès votre arrivée pour une efficacité maximale.

Acceptable Short-Term Storage:

• These components can be kept at room temperature (15-25°C) pour jusqu'à 12 weeks without impacting their performance.

Storing Remaining Components:

• All other kit components can be stored dry at room temperature (15-25°C).
• They will remain stable for at least 18 mois dans ces conditions.

Important Notes:

• If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
• Always ensure all buffers reach room temperature before using them for optimal results.

Données d'expérimentation