introduzione
This kit empowers researchers with a streamlined solution for isolating high-quality circulating cell-free DNA (cfDNA) from various biofluids:
- Sample Types: Plasma, Siero (1-6ml)
- Target: cfDNA molecules
- Downstream Applications:
- PCR (Reazione a catena della polimerasi)
- Real-time PCR
- Biochip analysis
- Next-generation sequencing (NGS)
Specifiche
| Caratteristiche | Specifiche |
| Funzioni principali | Isolation circulating DNA from 1-6ml plasma, siero, fluidi corporei |
| Applicazioni | qPCR, NGS, eccetera. |
| Tecnologia di purificazione | Tecnologia delle perle magnetiche |
| Metodo di processo | Manuale o automatico |
| Tipo di campione | Siero, plasma |
| Importo del campione | 1-6ml |
| Volume di eluizione | ≥50 μl |
| Tempo per corsa | ≤60 minuti |
Principio
This product utilizes innovative magnetic bead technology for efficient DNA extraction:
- Lysis and Digestion: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, Rilasciare il DNA nel lisato.
- Binding to Magnetic Beads: Magnetic particles coated for high DNA affinity are added to the lysate. DNA molecules selectively bind to the surface of these beads, while impurities like proteins remain unbound.
- Washing and Purification: A series of washes with different solutions remove unbound proteins and other cellular debris.
- Elution: A special elution buffer disrupts the interaction between the DNA and the magnetic beads, allowing the purified DNA to be collected in a separate tube.
Vantaggi
- Economia – meno di 50% del prezzo di Qiagen e di altri prodotti importati
- Automatico – without labor
- Purezza elevata – DI 260 / 280: 1.7-1.9, DI 260 / 230:1.5-2.0
Contenuto del kit
| Contenuti | IVD5435 |
| Tempi di purificazione | 50 |
| Particelle MagPure F | 14 ml |
| RNA portante | 310 μg |
| Proteinasi K | 240 mg |
| Tampone di dissoluzione della proteasi | 15 ml |
| Respingente Scheda di sicurezza | 15 ml |
| Buffer MLK | 500 ml |
| Buffer MAW1 | 250 ml |
| Buffer MW2* | 50 ml |
| Tampone di eluizione | 60 ml |
Conservazione e stabilità
Ideal Storage:
- Store Proteinase K, RNA portante, and MagPure Particles F in the refrigerator (2-8°C) upon arrival for maximum effectiveness.
Acceptable Short-Term Storage:
- These components can be kept at room temperature (15-25°C) for up to 12 weeks without impacting their performance.
Storing Remaining Components:
- All other kit components can be stored dry at room temperature (15-25°C).
- They will remain stable for at least 18 mesi in queste condizioni.
Important Notes:
- If storing the entire kit at 2-8°C, remember to redissolve buffers before use.
- Always ensure all buffers reach room temperature before using them for optimal results.
Dati dell'esperimento
Recensioni
Non ci sono ancora recensioni.