Introduction
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, tissus, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-RAP, Transfert du Nord, ARN poly A+ (ARNm) purification, protection contre les nucléases, et traduction in vitro.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Co-isolation DNA and RNA /protein from a single sample (cellules, mouchoir doux, échantillon de plante) |
| Applications | FDS-PAGE electrophoresis and western blot, etc. |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Culture cells, animal tissues, plant fungi, levure, bacteria and other samples |
| Montant de l'échantillon | Cultured cells: < 10^7
Tissu animal: ≤ 20 mg Plant samples: ≤ 150 mg Yeast cells: 2 X 10^6 – 5 X 10^7 |
Principe
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
Avantages
- Haute qualité – high purity total RNA / DNA can be directly used in a variety of downstream applications
- Rapide – column method can complete the extraction of several samples in 30 minutes
- Sûr – no phenol chloroform extraction required
- Simultaneous extraction- simultaneously isolate DNA and RNA from one sample
Contenu du kit
| Contenu | R521102 | R521103 |
| Temps de purification | 50 Préparations | 250 Préparations |
| Mini-colonnes HiPure DNA | 50 | 250 |
| Mini-colonnes HiPure ARN | 50 | 250 |
| 2Tubes de prélèvement ml | 100 | 2 X 250 |
| Buffer RLC | 50 ml | 200 ml |
| Tampon GW1* | 22 ml | 66 ml |
| Tampon RW1 | 50 ml | 200 ml |
| Tampon RW2* | 50 ml | 3 X 50 ml |
| Eau sans RNase | 10 ml | 30 ml |
| Tampon d'élution | 10 ml | 30 ml |
| Buffer ALO(5%FDS) | 10 ml | 30 ml |
Stockage et stabilité
HiPure Kit can be stored dry at room temperature (15–25°C) et sont stables pendant au moins 18 mois dans ces conditions. Pendant l'expédition, crystals or precipitation may form in the Buffer RLC. Dissoudre en réchauffant le tampon à 37°C.
Commentaires
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