介紹
The Kit provides fast purification of high-quality DNA, RNA and Prote from cells, 組織, and yeast using silica-membrane spin columns with a binding capacity of 100ug RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with isopropanol or LiCl are eliminated. RNA purified using the HiPure Total RNA Purification System is ready for applications such as RT-聚合酶鍊式反應, 北方印跡, 多聚A+RNA (信使RNA) 純化, 核酸酶保護, 和體外翻譯.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Co-isolation DNA and RNA /protein from a single sample (細胞, 軟組織, 植物樣本) |
| 應用領域 | 安全資料表-PAGE electrophoresis and western blot, ETC |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | Culture cells, 動物組織, plant fungi, 酵母菌, bacteria and other samples |
| 樣品量 | 培養細胞: < 10^7
動物組織: ≤ 20 毫克 Plant samples: ≤ 150 毫克 Yeast cells: 2 X 10^6 – 5 X 10^7 |
原則
The Kit isolates total RNA from up to 10 7 cells or 30 mg tissue. A short workflow enables RNAisolation with genomic DNA removal in less than 25 分分鐘. 首先將樣品裂解並均質化. The lysate is passed through a DNA Mini column, ethanol is added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30 µl water using the Kit.
優點
- 高品質 – 高純度總RNA / DNA可以直接用於多種下游應用
- 快速地 – 列方法可以完成在中提取幾個樣品 30 分分鐘
- 安全的 – 無需苯酚氯仿萃取
- 同時提取- 同時將DNA和RNA與一個樣品分離
套件內容
| 內容 | R521102 | R521103 |
| 淨化時間 | 50 準備工作 | 250 準備工作 |
| HiPure DNA 迷你柱 | 50 | 250 |
| HiPure RNA 迷你柱 | 50 | 250 |
| 2毫升收集管 | 100 | 2 X 250 |
| 緩衝RLC | 50 毫升 | 200 毫升 |
| 緩衝區GW1* | 22 毫升 | 66 毫升 |
| 緩衝器RW1 | 50 毫升 | 200 毫升 |
| 緩衝器 RW2* | 50 毫升 | 3 X 50 毫升 |
| 無核糖核酸酶水 | 10 毫升 | 30 毫升 |
| 洗脫緩衝液 | 10 毫升 | 30 毫升 |
| Buffer ALO(5%安全資料表) | 10 毫升 | 30 毫升 |
儲存和穩定性
HiPure Kit can be stored dry at room temperature (15–25°C) 並且至少穩定 18 在這些條件下幾個月. 裝運期間, crystals or precipitation may form in the Buffer RLC. 將緩衝液加熱至 37°C 溶解.
評論
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