HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, RAP, séquençage,digestion des restrictions, or various labeling reactions. En outre, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Détails
Caractéristiques
| Caractéristiques | Caractéristiques |
| Fonctions principales | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Applications | RAP, séquençage, labeling reactions, ligations and restriction digestion, etc.. |
| Méthode de purification | Mini colonne de rotation |
| Technologie de purification | Technologie de la silice |
| Méthode de processus | Manuel (centrifugation ou vide) |
| Échantillon type | Crude DNA, enzymatic reaction solution |
| Montant de l'échantillon | <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”><150µl |
| Récupération | 80% |
| Volume d'élution | <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>≥15μl |
| Temps par course | <portée “=”” style=”contour: 0px; dimensionnement de la boîte: bordure-boîte; taille de police: 16px;”>≤10 minutes |
| Volume de liquide transporté par colonne | 800µl |
| Rendement de liaison de la colonne | 20µg |
Principe
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Avantages
- High recovery efficiency – jusqu'à 80% DNA recovery
- Rapide – isolation can be completed in 10 minutes by column gel method
- Low elution volume – elution volume can be as low as 10μl
- Efficient – efficient removal of various inhibitory factors
Contenu du kit
| Contenu | D214102 | D214103 |
| Temps de purification | 100 Préparations | 250 Préparations |
| Buffer GWP | 30 ml | 60 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Tampon d'élution | 30 ml | 60 ml |
| Mini-colonnes HiPure DNA I | 50 | 250 |
| 2 Tubes de prélèvement ml | 50 | 250 |
Stockage et stabilité
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
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