HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sekvenciranje,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
pojedinosti
Tehnički podaci
| Značajke | Tehnički podaci |
| Glavne funkcije | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Prijave | PCR, sekvenciranje, labeling reactions, ligations and restriction digestion, itd. |
| Metoda pročišćavanja | Mini spin stupac |
| Tehnologija pročišćavanja | Silika tehnologija |
| Procesna metoda | Priručnik (centrifugiranje ili vakuum) |
| Vrsta uzorka | Crude DNA, enzymatic reaction solution |
| Količina uzorka | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”><150μl |
| Oporavak | 80% |
| Volumen eluacije | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≥15μl |
| Vrijeme po vožnji | <span “= =”” style=”outline: 0px; box-sizing: border-box; font-size: 16px;”>≤10 minutes |
| Nosivi volumen tekućine po stupcu | 800µl |
| Prinos vezivanja kolone | 20µg |
Načelo
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Prednosti
- High recovery efficiency – do 80% DNA recovery
- Brzo – isolation can be completed in 10 minutes by column gel method
- Low elution volume – elution volume can be as low as 10μl
- Efficient – efficient removal of various inhibitory factors
Sadržaj kompleta
| Sadržaj | D214102 | D214103 |
| Vremena pročišćavanja | 100 Pripreme | 250 Pripreme |
| Međuspremnik GWP | 30 ml | 60 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elucijski pufer | 30 ml | 60 ml |
| HiPure DNA mini stupci I | 50 | 250 |
| 2 ml cijevi za prikupljanje | 50 | 250 |
Skladištenje i stabilnost
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. Ako se u puferima stvori bilo kakav talog, zagrijte na 37 ℃ da se otopi.
Recenzije
Još nema recenzija.