This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
細節
規格
| 特徵 | 規格 |
| 主要功能 | Isolation total DNA from tissue using economic salt out method |
| 應用領域 | 聚合酶鍊式反應, 酵素消化, Southern hybridization,ETC. |
| 淨化技術 | Salting out method |
| 工藝方法 | 手動的 (離心或真空) |
| 樣品類型 | 動物組織 |
| 樣品量 | Unlimited |
| 洗脫體積 | ≥100μl |
| 每次運行時間 | Variation with sample amount |
原則
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, such as proteins, are removed by salt precipitation. 最後, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 和 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20℃, or -80°C.
優點
- 安全的 – without phenol chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- 高純度 – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
套件內容
| 內容 | D331201 | D331202 | D331203 |
| 淨化時間 | 1 G | 5 G | 50 G |
| 蛋白酶K | 330 微升 | 1.8 毫升 | 18 毫升 |
| Buffer WTL | 33 毫升 | 160 毫升 | 2 X 800 毫升 |
| Buffer PPS | 12 毫升 | 55 毫升 | 500 毫升 |
| 核糖核酸酶溶液 | 330 微升 | 1.8 毫升 | 18 毫升 |
| 緩衝區TE | 12 毫升 | 60 毫升 | 200 毫升 |
儲存和穩定性
RNase Solution should be stored at 2-8°C upon arrival. 然而, 短期儲存 (取決於 24 週) 在室溫下 (15-25℃) 不影響他們的表現. 其餘套件組件可在室溫下乾燥保存 (15-25℃) 並且至少穩定 18 在這些條件下幾個月. 整個套件可在 2-8°C 下保存, 但在這種情況下，緩衝液應在使用前重新溶解. 使用時確保所有緩衝液均處於室溫.
評論
還沒有評論.