This product is designed for purification of high-molecular-weight genomic or mitochondrial DNA from a variety of tissue and culture cells. The convenient, scalable purification procedure removes contaminants and enzyme inhibitors such as proteins and divalent cation, and purified DNA is ready for immediate use in sensitive downstream applications or for archiving.
Einzelheiten
Spezifikationen
| Merkmale | Spezifikationen |
| Hauptfunktionen | Isolation total DNA from tissue using economic salt out method |
| Anwendungen | PCR, Enzymverdauung, Southern hybridization,usw. |
| Reinigungstechnologie | Salting out method |
| Prozessmethode | Handbuch (Zentrifugation oder Vakuum) |
| Beispielstyp | Animal tissue |
| Probenmenge | Unlimited |
| Elutionsvolumen | ≥100μl |
| Zeit pro Lauf | Variation with sample amount |
Principles
Cells are lysed with ananionic detergent in the presence of a DNA stabilizer. The DNA stabilizer limits the activity of intracellular DNases and also DNases found elsewhere in the environment. RNA is then removed by treatment with an RNA digesting enzyme. Other contaminants, wie Proteine, are removed by salt precipitation. Endlich, the genomic DNA is recovered by precipitation with alcohol and dissolved in Buffer TE. Purified DNA typically has an A260/A280 ratio between 1.7 Und 1.9, and is up to 200 kb in size. The DNA can be safely stored at 2-8°C, -20°C, or -80°C.
Vorteile
- Sicher – without phenol chloroform extraction
- Environment friendly – the reagents used are safe, non-toxic and without pollution
- High molecular weight – the molecular weight of genomic DNA is about 50-150kb
- Hohe Reinheit – the purified DNA has A260/280=1.8-1.9, A260/230=2.0-2.5
- Unlimited sample size – solution type operation, sample volume can be adjusted at will
- Cost performance – the most economical nucleic acid extraction technology
Inhalt des Kits
| Inhalt | D331201 | D331202 | D331203 |
| Reinigungszeiten | 1 G | 5 G | 50 G |
| Proteinase K | 330 µl | 1.8 ml | 18 ml |
| Buffer WTL | 33 ml | 160 ml | 2 X 800 ml |
| Buffer PPS | 12 ml | 55 ml | 500 ml |
| RNase-Lösung | 330 µl | 1.8 ml | 18 ml |
| Buffer TE | 12 ml | 60 ml | 200 ml |
Lagerung und Stabilität
RNase Solution should be stored at 2-8°C upon arrival. Jedoch, kurzfristige Lagerung (bis zu 24 Wochen) bei Raumtemperatur (15-25°C) hat keinen Einfluss auf ihre Leistung. Die restlichen Kit-Komponenten können trocken bei Raumtemperatur gelagert werden (15-25°C) und sind mindestens stabil 18 Monate unter diesen Bedingungen. Das gesamte Kit kann bei 2–8 °C gelagert werden, In diesem Fall sollten die Puffer jedoch vor der Verwendung erneut aufgelöst werden. Stellen Sie sicher, dass alle Puffer bei der Verwendung Raumtemperatur haben.
Rezensionen
Es gibt noch keine Rezensionen.