規格
SENO Taq DNA 聚合酶 5000 單位 (shipping with icebag)
筆記: This product is liquid texture, the lyophilized powder is not provided.
產品描述:
- Pure DNA Polymerase with a molecular weight of 94 肯德基.
- Possesses 5′-3′ DNA polymerase and 5′-3′ 核酸外切酶活性, but lacks 3′-5′ 核酸外切酶活性.
- Contains 10x 聚合酶鍊式反應 Buffer for direct loading, enhancing experimental efficiency.
- Extension speed of 1-2 kb/min.
- DNA template has A at the 3′ 結尾, facilitating direct cloning with TA vector.
反應系統
- 純DNA聚合酶: 0.8微升 (adjust for amplification needs)
- 10X PCR緩衝區: 2微升
- 脫氧核糖核酸 (2.5毫米): 2微升
- Primerp: 0.1-1微升 (10-50pmol)
- firstr: 0.1-1微升 (10-50pmol)
- 模板: <1微克
- 水: 最多20μl
週期計劃:
| 步 | 溫度 | 時間 |
|---|---|---|
| 初始變性 | 94℃ | 3分分鐘 |
| 變性 | 94℃ | 30s |
| 退火 | 45-60℃ | 30s |
| 擴大 | 72℃ | 30S-2min |
| 最終延期 | 72℃ | 10分分鐘 |
結果檢測:
- After PCR completion, take 5μl of product for agarose gel electrophoresis.
- Direct detection by loading samples without mixing with Loading Buffer.
Scope of Application:
- PCR amplification of DNA fragments, DNA標記, 底漆擴展, 序列確定, 鈍端添加.
- Suitable for T/A vector cloning.
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