HiPure 循環 DNA Midi Spin 試劑盒 D (自旋協定)

介紹

HiPure Circulating DNA Midi Kit, designed to efficiently purify short fragments of cell-free DNA (遊離DNA) from human biofluids for clinical use:

• 目標:
  • 血液中腫瘤來源的 cfDNA 片段和 mRNA
  • 母血中的胎兒核酸
• Sample Types:
  • 人類血漿
  • 人血清
  • 人類尿 (fresh or frozen once)
• 專注於短DNA: Captures short nucleic acids (<1000BP) 常見於生物體液中.
• 高效淨化: Effectively recovers cfDNA from various biofluids.
• Clinical Applications: Purified cfDNA is suitable for approved in vitro diagnostic (體外診斷) tests.

好處:

• 針對短 cfDNA 進行了優化: Ideal for capturing short DNA fragments relevant for cancer biomarkers and liquid biopsies.
• 廣泛的樣品相容性: Works with various human biofluids, 提供樣本選擇的靈活性.
• Larger Scale Purification: The Midi Kit format allows the processing of larger sample volumes compared to Mini Kits, potentially increasing cfDNA yield.
• Clinical Utility: Purified DNA can be directly used in approved clinical IVD tests.

This kit empowers researchers and clinical laboratories studying circulating DNA for applications in oncology, 非侵入性產前檢測 (NIPT), and liquid biopsies, ultimately aiding in patient diagnosis and management.

細節

規格

原則

This product utilizes silica gel columns for efficient DNA extraction:

1. Lysis & Digestion: The sample undergoes treatment with a special solution and enzyme to break open cells and degrade proteins, releasing DNA.
2. Binding to Silica: The mixture is transferred to a column. The silica membrane in the column captures DNA molecules, while other substances pass through.
3. 洗滌: Wash buffers to remove leftover proteins and cellular debris.
4. 洗脫: A low-salt buffer elutes the purified DNA from the silica, collecting it in a separate tube.

此方法為需要純化 DNA 進行分析的各個領域的研究人員提供了寶貴的工具.

優點

• 高產 – 最優過程, 遊離DNA (>50BP) 可以最大程度地獲得
• 高濃度 – 低洗脫體積, 確保高核酸濃度
• 高純度 – 低醇結合法, 徹底去除抑制劑和蛋白質污染
• 高回收率 – 透過矽膠柱純化可在PG水平回收DNA

套件內容

儲存和穩定性

• 蛋白酶K & 載體RNA: Fridge (2-8℃) for best results.
• Everything Else: 室內溫度 (15-25℃) is fine.
• Important: If storing cold, warm buffers before use.