介紹
- 目標: Short DNA fragments (<1000BP) in the blood (tumor markers, fetal DNA) 或尿液.
- 效率: Purifies circulating nucleic acids from human biofluids (電漿, 血清, 尿).
- 應用領域: Extracted DNA is suitable for approved clinical diagnostic tests.
好處:
- Optimized for short DNA analysis.
- Works with various human fluids.
- A larger format processes more samples (potentially higher DNA yield).
- Directly usable in clinical tests.
This Midi Kit empowers researchers and clinical labs for applications in oncology, NIPT, and liquid biopsies.
規格
| 特徵 | 規格 |
| 主要功能 | 從 1-5ml 血漿中分離循環 DNA, 血清, and body fluids using vacuum protocol |
| 應用領域 | 定量PCR, 液體或固體晶片分析, hybridization SNP detection, ETC. |
| 純化方法 | 小型的 旋轉柱 |
| 淨化技術 | 二氧化矽技術 |
| 工藝方法 | 手動的 (真空) |
| 樣品類型 | 血清, 電漿, and other cell-free fluid samples |
| 樣品量 | 1-5毫升 |
| 洗脫體積 | ≥50μl |
| 每次運行時間 | ≤60分鐘 |
| 每根柱的載液量 | 4毫升 |
| 柱結合率 | 1毫克 |
原則
This product offers a streamlined method for isolating DNA using silica column technology:
- 裂解和消化: The sample undergoes treatment with a special solution (lysate) and an enzyme (protease) to break open cells and degrade proteins, 將DNA釋放到裂解物中.
- Binding to Silica: The lysate containing the DNA is transferred to a silica column. The silica membrane in the column selectively binds DNA molecules due to favorable interactions, 而蛋白質和其他雜質則透過.
- 洗滌: 洗滌緩衝液通過色譜管柱, 去除未黏附在二氧化矽上的未結合蛋白質和細胞碎片.
- 洗脫: A low-salt buffer is used to elute (release) the purified DNA from the silica membrane. 這會破壞 DNA 和二氧化矽之間的相互作用, 允許在單獨的管中收集純化的 DNA.
Key Advantages:
- 高效淨化: Silica column technology offers a fast and reliable process for isolating DNA from complex biological samples.
- High-Purity DNA: The selective binding properties of the silica membrane ensure the purification of high-quality DNA, suitable for various downstream applications (聚合酶鍊式反應, ETC。).
- 簡化的工作流程: The protocol involves minimal handling steps and centrifugation for user-friendly and efficient extraction.
此方法為需要純化 DNA 進行分析的各個領域的研究人員提供了寶貴的工具.
優點
- 高產 – 最優過程, 遊離DNA (>50BP) 可以最大程度地獲得
- 高濃度 – 低洗脫體積, 確保高核酸濃度
- 高純度 – 低醇結合法, 徹底去除抑制劑和蛋白質污染
- 高回收率 – 透過矽膠柱純化可在PG水平回收DNA
套件內容
| 內容 | 體外診斷3182 |
| 淨化時間 | 50 |
| 緩衝區ACL | 250 毫升 |
| 緩衝器 ACB* | 300 毫升 |
| 緩衝器 DCW1* | 22 毫升 |
| 緩衝器 DCW2* | 10 毫升 |
| 蛋白酶K | 540 毫克 |
| 蛋白酶溶解緩衝液 | 30 毫升 |
| 載體RNA | 110 微克 |
| 無核酸酶水 | 20 毫升 |
| HiPure CFDNA 迷你柱 | 50 |
| 2 毫升收集管 | 100 |
| 延長管 | 50 |
| 真空連接器 | 50 |
儲存和穩定性
- 蛋白酶K & RNA Carrier: Fridge (2-8℃) for best results.
- Other Kit Components: 室內溫度 (15-25℃) is good.
- Storing Cold? Warm buffers up before use.
實驗數據
評論
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