Спецификация
| Атрибут | Подробности |
|---|---|
| Хранилище | 2-8℃ |
| Единица | Коробка |
| Срок действия | 6 месяцы |
| Английское имя | Pepsase Activity Assay Kit |
| Метод обнаружения | Спектрофотометр/считыватель микропланшета |
| Спецификация | 100Т/48С |
Состав продукта
| Название реагента | Спецификация | Условие хранения |
|---|---|---|
| Решение для экстракции | Жидкость 50 мл×1 флакон | Хранить при 4℃ |
| Реагент Один | Powder×1 bottle | Хранить при 4℃ |
| Реагент второй | Жидкость 15 мл×1 флакон | Хранить при 4℃ |
| Реагент Три | Powder×1 bottle | Хранить при 4℃ |
Instructions for preparing the solutions:
- Solution One: Добавлять 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: Добавлять 10 mL of distilled water before use and dissolve completely.
Описание продукта:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- Adjustable pipette
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Ice
- Дистиллированная вода
Примечание: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
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