Specification
| Attribute | Details |
|---|---|
| Storage | 2-8℃ |
| Unit | Box |
| Validity Period | 6 months |
| English Name | Pepsase Activity Assay Kit |
| Detection Method | Spectrophotometer/Microplate Reader |
| Specification | 100T/48S |
Product Composition
| Reagent Name | Specification | Storage Condition |
|---|---|---|
| Extraction Solution | Liquid 50 mL×1 bottle | Store at 4℃ |
| Reagent One | Powder×1 bottle | Store at 4℃ |
| Reagent Two | Liquid 15 mL×1 bottle | Store at 4℃ |
| Reagent Three | Powder×1 bottle | Store at 4℃ |
Instructions for preparing the solutions:
- Solution One: Add 10 mL of Reagent Two before use and dissolve completely.
- Solution Three: Add 10 mL of distilled water before use and dissolve completely.
Product Description:
- Pepsin is secreted by the chief cells of the gastric mucosa and hydrolyzes proteins in food into small peptides. It is commonly used for the identification of hypochlorhydria, and reduced secretion of pepsin may also occur in conditions such as chronic gastritis, chronic gastric dilation, and chronic duodenitis.
- Pepsin catalyzes the hydrolysis of hemoglobin, and the hydrolysis product tyrosine exhibits a characteristic absorption peak at 275nm. Enzyme activity is calculated by measuring the changes in absorbance.
Instruments and supplies required:
- UV spectrophotometer/enzyme marker
- Benchtop centrifuge
- Water bath
- Adjustable pipette
- Micro quartz cuvette/96-well UV plate
- Mortar and pestle
- Ice
- Distilled water
Note: It is recommended to perform a pilot experiment with 2-3 samples showing significant expected differences before conducting the actual experiment. If the absorbance values of the samples are not within the measurement range, dilution or increasing the sample volume for detection is recommended.
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