Солнечный протеиновый сборный гель (Родной) 8-20%, 15 колодцы

A Protein Precast-Gel (Родной) 8-20%, 15 wells is a pre-made polyacrylamide gel designed for the separation of native proteins based on their size without denaturation. These gels are commonly used in techniques like native polyacrylamide gel electrophoresis (СТРАНИЦА) or blue native PAGE.

Features of Protein Precast-Gel (Родной) 8-20%, 15 колодцы:

1. Gradient Composition: The gel has a gradient composition of acrylamide, обычно варьируется от 8% к 20%, which allows the separation of proteins over a broad size range.
2. 15 Уэллс: The gel is precast with 15 колодцы, providing the user the ability to load and separate multiple protein samples simultaneously.
3. Native Conditions: The gel is designed to maintain native conditions, preserving the native structure and activity of proteins.

How to Use Protein Precast-Gel (Родной) 8-20%, 15 колодцы:

1. Приготовление геля:
   • Remove the Protein Precast-Gel from its packaging. Place the gel into the gel-running apparatus or electrophoresis tank, ensuring proper alignment with the electrodes.
2. Загрузка образца:
   • Prepare your protein samples by mixing them with a suitable loading buffer that is compatible with native electrophoresis. Load the protein samples into the wells of the gel using a micropipette.
3. Электрофорез:
   • Submerge the gel in the electrophoresis buffer in the electrophoresis tank. Apply an electric current according to the manufacturer’s recommendations. Native electrophoresis typically runs at a lower voltage compared to denaturing gels.
4. Run Duration:
   • Run the electrophoresis until the proteins have migrated sufficiently through the gel based on the desired resolution. The run time will depend on the specific apparatus and conditions used.
5. Gel Staining:
   • После электрофореза, the gel can be stained to visualize the separated proteins. Common stains for native gels include Coomassie Brilliant Blue or silver staining.
6. Destaining and Imaging:
   • Destain the gel if necessary, and then image the gel using a gel documentation system or other appropriate imaging methods.
7. Анализ:
   • Analyze the gel to determine the molecular weights and relative quantities of the separated proteins. Compare the results with protein standards run on the same gel.